Mansoura University, Faculty of AgricultureJournal of Agricultural Chemistry and Biotechnology2090-3626111220201201Controlling Soft Rot Disease of Potatoes Caused by Enterobacter cloacae sub sp. dissolvens via Virulent Bacteriophages34535115269210.21608/jacb.2020.152692ENEman M.MareiDepartment of Agricultural Microbiology, Virology Laboratory, Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek- Shoubra 11241, Cairo, Egypt.A. A.EmbabyDepartment of Agricultural Microbiology, Virology Laboratory, Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek- Shoubra 11241, Cairo, Egypt.Sohair I.El-AfifiDepartment of Agricultural Microbiology, Virology Laboratory, Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek- Shoubra 11241, Cairo, Egypt.Journal Article20210301<em>Enterobacter cloaca</em> causes bacterial soft rot disease of potatoes<strong>.</strong> Bacteriophages can be used as a biocontrol agent against pathogenic bacteria. The possibility of using lytic phage isolates to control soft rot disease of potato was studied.Isolation and identification ofdifferent virulent bacteriophages specific to <em>Enterobacter cloacae </em>sub sp.<em> dissolvens</em> was took place by using biological and molecular techniques. The efficiency of lytic phage isolates on <em>E. cloacae </em>sub sp.<em> dissolvens</em> as a biocontrol agent under greenhouse condition was evaluated.Thetwo virulent phage isolates specific to <em>E. cloacae </em>sub sp.<em> dissolvens</em> were isolated, identified and designated as EdIv-1 and EdIv-2. Mixture of the two virulent phages (EdIv-1 and EdIv-2) could be used as a bio control agent against <em>E. cloacae </em>sub sp.<em> dissolvens</em> in soil. The data indicated that the number of bacterium in the soil treated with this treatment as a cocktail of phages were considerably lower than in the soil treated with bacterium only in all the different periods and other treatments. In addition the highest percentage of decreased plants was recorded after 30 days (71%). Virulent phage isolates were found to be stable, infecting <em>E. cloacae </em>sub sp.<em> dissolvens. </em>It could be concludedthat cocktail of phages can be used as effective biological control agent against the plant pathogenunder the environmental condition in Egypt.https://jacb.journals.ekb.eg/article_152692_b4f1977e91816845d4f788f990274f56.pdfMansoura University, Faculty of AgricultureJournal of Agricultural Chemistry and Biotechnology2090-3626111220201201Genetic Relationships and Diversity among Pea (Pisum sativum L.) Genotypes Assessed using Agro-Morphological and Molecular Markers35336313730510.21608/jacb.2020.137305ENB. E.S.Abd El-FatahDepartment of Genetics, the Faculty of Agriculture, Assiut University Assiut 71526, EgyptDina S.NafeaDepartment of Vegetables, the Faculty of Agriculture, Assiut University Assiut 71526, EgyptJournal Article20210109Fifteen pea (<em>Pisum sativum</em> L.) genotypes were evaluated for their agro-morphological performance and molecular diversity during 2017-2018 and 2018-2019 seasons. Combined analysis of variance revealed that there are highly significant differences among pea genotypes tested for all agro-morphological traits studied. The phenotypic coefficients of variation (PCV) values were relatively greater than Genotypic coefficients of variation (GCV) for all traits; however, GCV values were near to PCV values for the traits, like plant height, pods/plant, 100 seeds weight and pod width. High heritability coupled with high genetic advance was obtained for, plant height, reflecting the presence of additive gene action for the expression of this trait. High to moderate heritability coupled with moderate genetic gain were exhibited for number of pods per plant, 100 seeds weight, pod length, seeds per pod, pod weight and number of branches per plant. The Euclidean Distance among all genotypes based on agro-morphological traits was relatively wide. ISSR and SRAP markers were used to study the molecular diversity among pea genotypes. The molecular marker parameters revealed that SRAP markers were more efficient than ISSR markers with regards to polymorphism detection and in distinction among pea genotypes. Also, both ISSR and SRAP markers were able to amplify unique bands specific to a particular genotype. A positive and significant correlation (r=0.389: p ≤ 0.001) between ISSR and SRAP matrix was observed according to Mantel's test. Also, significant correlation (r = 0.411: p ≤ 0.001) between the matrices of combined molecular markers data and agro-morphological data.https://jacb.journals.ekb.eg/article_137305_b5734bdee1c7d52006ca989cc0ceada5.pdfMansoura University, Faculty of AgricultureJournal of Agricultural Chemistry and Biotechnology2090-3626111220201201Assessment of Heat Tolerance in Some Wheat Species and Interspecific Hybrids36537315270410.21608/jacb.2021.54316.1007ENM. A.El-RawyGenetics Department, Faculty of Agriculture, Assiut University, Assiut, EgyptAya AbdelredaGenetics Department, Faculty of Agriculture, Assiut University, Assiut, EgyptB. E. S.Abd El-FatahGenetics Department, Faculty of Agriculture, Assiut University, Assiut, EgyptMuhammad YoussefGenetics Dept., Faculty of Agriculture, Assiut UniversityJournal Article20210301Twelve accessions including two diploid, four tetraploid and six hexaploid genotypes were used in this study to assess heat tolerance in wheat. All possible crosses were made and the resulted hybrids with their parents were subsequently evaluated for heat tolerance in two sowing dates. Evaluation was based on 1000 grain weight, grain yield per plant, cell membrane thermostability and tetrazolium chloride reduction. Results revealed significant variability among wheat genotypes and hybrids in all evaluated traits under favorable and heat stress conditions. However, tetraploid genotypes showed the highest performance under heat stress followed by hexaploid and diploid genotypes, respectively. According to results of heat tolerance index, one tolerant and one susceptible parent from each ploidy level along with 6 related interspecific hybrids were chosen to be involved in molecular analysis. Target region amplification polymorphism (TRAP) markers were used based on fixed primers of heat shock protein genes. TRAP clearly confirmed the morpho-physiological findings and generated a dendrogram which was quietly like that of morpho-physiological data. TRAP was able to generate one and five specific bands for diploid and hexaploid genotypes respectively, while no specific bands were generated for tetraploid. In addition, the different genomes showed some shared bands between each other revealing their relationship. Interestingly, two specific bands for tolerant genotypes of different ploidy level were generated which were absent in all susceptible genotypes. Findings herein are of high importance and could help in successive breeding programs for wheat improvement.https://jacb.journals.ekb.eg/article_152704_607e85e50357a7068425308f684eaead.pdfMansoura University, Faculty of AgricultureJournal of Agricultural Chemistry and Biotechnology2090-3626111220201201Molecular Markers, Yield Performance and Berry Sensory Attributes in 10 Grape Cultivars Cultivated in Assiut Governorate37538215271210.21608/jacb.2021.58871.1010ENKaram AAmeinDepartment of Genetics, Faculty of Agriculture, Assiut UniversityR. A.IbrahimDepartment of Pomology, Faculty of Agriculture, Assiut University, Assiut 71526, Egypt.M. M.ShaabanDepartment of Pomology, Faculty of Agriculture, Assiut University, Assiut 71526, Egypt.Shamia A.ThabetDepartment of Pomology, Faculty of Agriculture, Assiut University, Assiut 71526, Egypt.A. K. A.MohamedDepartment of Pomology, Faculty of Agriculture, Assiut University, Assiut 71526, Egypt.Journal Article20210301Yield performance and berry sensory attributes in 10 grape cultivars were studied under Assiut conditions. In addition, the genetic variability and relationships among these cultivars were determined based on RAPD and SRAP molecular markers. Genetic variations were observed between the tested cultivars in all studied traits of yield and yield components as well as in berry sensory attributes. 100 sites of grape genome were amplified by RAPD and SRAP primers and used to study the interrelationships between 10 grape cultivars. The highest interrelationships were found between “Black Muscat and Provano”, “Palomino and Bez El-Naka”, and between “Palomino and Ruby Seedless”. The lowest relatedness was found between Beauty Seedless and the other nine grape cultivars. The phenotypic data and molecular markers were effective in estimating the genetic variability between grape cultivars. The study indicated the presence of abundant genetic variability among some of the important commercial grape cultivars. Significant positive correlation was found between the phenotypic and genotypic distance indicated that the studied RAPD and SRAP markers were able to bind to effective regions in the genome.https://jacb.journals.ekb.eg/article_152712_c4b050e9e1a0da5c7b6080dce9ecaff0.pdfMansoura University, Faculty of AgricultureJournal of Agricultural Chemistry and Biotechnology2090-3626111220201201Heterosis and Combining ability Estimates using Line x Tester Analysis to Develop Wide Compatibility and Restorer Lines in Rice38339316116610.21608/jacb.2020.161166ENM. M. A.Awad-AllahRice Res. Dept. Field Crops Res. Inst., ARC, EgyptJournal Article20210405This investigation was conducted at Agriculture Research Station, Sakha, Kafr El-Sheikh, Egypt, during 2017 and 2018 seasons to study combining ability, gene action, heterosis, and degree of dominance for grain yield and its contributing traits in some wide compatibility lines. Eight genotypes included the three lines; O2428-P-7-7, Norti, Gz 5121-5-2 as female parents with five testers Dular, Nekken 1, Giza 178, Gz 5934-7-2-3 and IR 25571-31-1, are used in a line X testers cross. The analysis of variance revealed significant differences among parents, crosses and line x tester interaction for all studied traits except panicle weight in parents. The ratio of K<sup>2</sup> GCA / K<sup>2</sup> SCA was more than unity for all studied traits indicated preponderance of additive gene effects in the expression of these traits except panicle weight. Rice varieties; Dular and Nekken 1 can be identified as wide compatible lines. Most of F<sub>1</sub> crosses showed significant values in the heterosis for all studied traits. The crosses Norti X Nekken 1 and Norti X Giza 178 showed the highest values for mean performance and the three types of heterosis for grain yield. In addition, the highest significant positive values of specific combining ability were shown in the four cross combinations O2428-P-7-7 X IR25571-31-1, Gz 5121-5-2 X Gz 5934-7-2-3, Gz 5121-5-2 X Dular and Norti X Giza 178. The results of this study indicate that the parental lines can be used for improvement and utilized in hybrid rice breeding program.https://jacb.journals.ekb.eg/article_161166_ce4b06ff7fddebfae425136eea226134.pdf