Mansoura University, Faculty of AgricultureJournal of Agricultural Chemistry and Biotechnology2090-362612420210401Use of Trichoderma Spp. For Biological Control of Sugar Beet Damping- Off Caused by Fusarium Spp.757716667110.21608/jacb.2021.166671ENA. Z. A.AshourPlant Pathology Research Institute, Agricultural Research Center (ARC), Giza, Egypt.A. M.El-SawahDept. Microbiology, Fac. Agri. Mansoura Univ., Mansoura, Egypt.Aida H.AfifyDept., Microbiology, Fac., Agric., Mansoura Univ., Mansoura , EgyptJournal Article20210311Antagonism between biocontrol agents <em>Trichoderma viride, </em><em>T. </em><em>harzianum </em>and <em>T. hamatum </em>against three phytopathogenic fungi: <em>Fusarium solani, </em><em>F. </em><em>oxysporum </em>and <em>F. </em><em>dimerum </em>which cause damping-off on sugar beet cultivars in Egypt. <em>In vitro</em>, percentage reduction of <em>T. </em><em>viride </em>was more than the other fungi for the untreated with <em>Fusarium solani </em>and <em>F. </em><em>oxysporum, </em>while <em>Trichoderma hamatum </em>was effective antagonist for the control of <em>F. </em><em>dimerum</em>. <em>In vivo</em>, <em>T. </em><em>viride </em>was the most antagonist effective for untreated with <em>F. solani (</em>85%) survival plants<em>, </em>while <em>T. hamatum </em>was the most antagonist effective for the control of <em>F.</em><em>oxysporum</em>and <em>F. dimerum </em>(87.5, 82.5) %survival plants. This result shows that <em>Trichoderma </em>spp. are very effective biocontrol agents against <em>Fusarium </em>spp. involved in sugar beet damping-off.https://jacb.journals.ekb.eg/article_166671_45c38d93ba815d6bb137f637a05bec7a.pdfMansoura University, Faculty of AgricultureJournal of Agricultural Chemistry and Biotechnology2090-362612420210401Application of Some Organic Farming Methods to Enhancement The Growth and Production of Green Onion798916261610.21608/jacb.2021.162616ENH. AbdelrahmanAgricultural Microbiology Department, Faculty of Agriculture, Benha University, Moshtohor, Qalyoubeia 13736, EgyptR. A.ZaghloulAgricultural Microbiology Department, Faculty of Agriculture, Benha University, Moshtohor, Qalyoubeia 13736, EgyptH. A.Abou-AlyAgricultural Microbiology Department, Faculty of Agriculture, Benha University, Moshtohor, Qalyoubeia 13736, EgyptA. A.RagabCentral Lab. of Organic Agriculture, Agricultural Research Center, Giza, EgyptM. M. K.ElmaghrabyCentral Lab. of Organic Agriculture, Agricultural Research Center, Giza, EgyptJournal Article20210305<em>In vitro</em> antagonistic effect of various PGPR namely <em>A. chroococcum</em>, <em>B. megaterium</em>, <em>B. circulans</em>, <em>B. subtilis, Ps. fluorescens</em> and<em> T. viride </em>against <em>F. solani </em>or<em> R. solani </em>was determined. Results observed that using all PGPR strains suppressed <em>F. solani </em>and<em> R. solani</em>. The inhibition zone increased by increasing incubation time. Under greenhouse condition, onion inoculation with investigated PGPR strains reduced disease severity of <em>F. solani</em> by rate ranging between (79.4-93.5%). However, they reduce disease severity of <em>R. solani</em> by rate ranging between (84.5-93.6%). Accordingly, the rate of disease incidence reduced by the range between (68.4-81.8%) and (65.5-81.8%) in case of infested with <em>F. solani</em> and <em>R. solani </em>respectively. Moreover, they had positive effect on plant defense enzymes, nutrients uptake and growth parameters. In farm of the Central Laboratory of Organic Agriculture, Giza station, ARC Giza, Egypt, the open field experiment was carried out to study the integrated effect of biofertilizing-PGPR and biocontrol agents with compost to improve green onion growth characteristics and yield under the organic agriculture system. During the experiment the soil microbial enzymes activity, total NPK and plant uptake, defense enzymes activity, growth characteristics and vegetative yield were estimated. Data obtained showed that onion treated with compost + biofertilizing-PGPR +<em> B</em>. <em>subtilis</em> +<em> T. viride </em>(T7) gave the highest values of in all estimated parameters except defense enzymes activity. So, it can be recommended as integrated fertilizing program to promote green onion growth, increase crop production, and decrease production costs.https://jacb.journals.ekb.eg/article_162616_66d673205d70943e114d2f29b007ef54.pdfMansoura University, Faculty of AgricultureJournal of Agricultural Chemistry and Biotechnology2090-362612420210401Bacteriological Quality of Drinking Water at Different Cities in Egypt919616871510.21608/jacb.2021.168715ENMai Abd-elhameedMicrobiology Dept ., Fac. of Agric ., Mansoura Univ., Mansoura , Egypt .M. A.E. SelimMicrobiology Dept ., Fac. of Agric ., Mansoura Univ., Mansoura , Egypt .Aida H.AfifyDept., Microbiology, Fac., Agric., Mansoura Univ., Mansoura , EgyptF. I.A. HaukaMicrobiology Dept ., Fac. of Agric ., Mansoura Univ., Mansoura , Egypt .Journal Article20210313Bacteriological examinations were carried on four types of water samples which collected from tap water, river Nile water, ground water and bottled water, at different cities in Dakahlia and Damietta Governorates, Egypt during 2017/2018 in order to determine the level and total count of bacterial isolates were present in our water collected samples . These bacterial groups were described as coliform bacteria, fecal coliform bacteria, and fecal streptococci, according to total bacterial counts methods. Generally,the highest number of total bacteria was found in Damietta, after that the number of total bacteria were presented in Sherbin. On the other hand, coliform bacteria were found to be lower counted than Mansoura water samples.https://jacb.journals.ekb.eg/article_168715_a3f3e6677af79efbbd355992fc7c774c.pdfMansoura University, Faculty of AgricultureJournal of Agricultural Chemistry and Biotechnology2090-362612420210401Suppression of Sclerotium cepivorum Using Biofumigation By Glucosinolate-Containing Plant and Cyanogenic Bacteria9710617126010.21608/jacb.2021.171260ENH. M.AbdelrahmanAgricultural Microbiology Department, Faculty of Agriculture, Benha University, Moshtohor, Qalyoubeia 13736, EgyptR. M.ElmeihyAgric. Microbiology Dept., Fac. Agric. Moshtohor, Benha Univ., EgyptA. A.SalemAgric. Microbiology Dept., Fac. Agric. Moshtohor, Benha Univ., EgyptJournal Article20210315In dual culture antagonism test, six of eight cyanogenic bacteria were able to reduce the growth of <em>Sclerotium</em><em> cepivorum</em>. The strains <em>Glocoacetobacter diazotrophicus</em> and <em>Paenibacillus. polymyxa</em> GQ375783.1were the most effective bacteria that recorded the highest inhibition of <em>S. cepivorum</em> mycelial growth by 75% and 70% respectively. Based on HCN production, two strains recorded complete inhibition of fungal growth. Moreover, the ground tissues of the nasturtium plant were able to inhibit the mycelium growth of <em>S. cepivorum</em> by 100%. Results of the greenhouse experiment emphasized that white-rot incidence percentage recorded the lowest values in onion cultivated in soil amended with nasturtium plant and inoculated with <em>G. </em><em>diazotrophicus </em>plus <em>P. polymyxa</em> GQ375783.1<em>. </em>This treatment recorded the highest values of onion vegetative growth and their yield. Under field conditions, the soil treated with investigated two methods of biofumigation led to suppress white rot disease by the rate of 100% while using each one individually, it reduced disease by the rate of 94.4 and 94.9 % respectively. All onion treatments that inoculated with cyanogenic bacteria showed higher records of DH and N<sub>2</sub>-ase activity than uninoculated ones. The highest values DH and N<sub>2</sub>-ase were recorded with soil amended with nasturtium and inoculated with cyanogenic bacterial strains. Additionally, the same treatment gave the highest values of peroxidase and polyphenoloxidase activity, onion NPK content, and the plant's vegetative growth as well as their yield. Therefore, the biofumigation using nasturtium and investigated bacterial strains can be recommended in controlling White-rot disease in onions and increasing their productivity.https://jacb.journals.ekb.eg/article_171260_b165cc762b77b8c8f1770ccd5c5a56ff.pdf