Mansoura University, Faculty of AgricultureJournal of Agricultural Chemistry and Biotechnology2090-3626101120191101Effect of Probiotic Bacteria on Legumes Purees2092136316410.21608/jacb.2019.63164ENA. F. FathallahMicrobiology Dept. Fac. of Agriculture, Mansoura University, Mansoura, EgyptM. A. E. SelimMicrobiology Dept. Fac. of Agriculture, Mansoura University, Mansoura, EgyptAida H.AfifyDept., Microbiology, Fac., Agric., Mansoura Univ., Mansoura , EgyptFatma M. ElzamazamyNutrition and Food Sci., Home Economics, Fac. of Specific Education, Mansoura University, Mansoura, EgyptJournal Article20191101In this study three common legumes (Fababean, Chickpea and Soybean) were fermented using probiotic bacteria after isolated and identified by morphological, biochemical and molecular characteristics. The cultures containing <em>Lactobacillus acidophilus</em><em>, Streptococcus thermophilus</em> and <em>Bifidobacterium</em> <em>lactis </em>as probiotic bacteria were used to study their effect on the properties of some food products prepared from legumes. Results revealed that P Soy bean (puree) recorded the highest scores in appearance (8.5 ± 0.2), acceptable odor and mouth feeling scores were (8.4 ± 0.2) respectively .However P Faba bean (puree) and P Chickpea (puree) had the best color (8.5 ± 0.2). While,taste was the best for P Faba bean (8.2 ± 0.2). Results indicated that the highest values of chemical parameters were recorded for P Faba bean in moisture and ash which were (4.7±0.1, (4.6±0.2) respectively. At the same time, P Soy bean in lipids had (19.0±0.0), and in protein was (39.3±0.1).Results obtained of P Chickpea in carbohydrates was( 62.6±0.1). Also, results obtained shows the effect of all three bacterial isolates <em>(Lactobacillus acidophilus , Bafidobacterhum lactis</em> and <em>Strentococcus thermophilus</em> ) as probiotic -fermented Faba bean, Chick pea and Soy bean. shows that ten storage days value at (5.5 ± 0.3) for the Soy bean (5.5± 0.3)for the Faba bean and (5.8 ± 0.5) for the Soy bean. Finaly the results concluded that the counts of probiotic bacterial isolates were improved the sensory and chemicaly evaluation of fermentated legumes when increased.https://jacb.journals.ekb.eg/article_63164_17a0ee8f6ff7e5cfbde5dea4b3baae51.pdfMansoura University, Faculty of AgricultureJournal of Agricultural Chemistry and Biotechnology2090-3626101120191101Molecular Assessment of Ten Faba Bean Genotypes Dissimilar in Bean Yellow Mosaic Virus Susceptibility2152206736610.21608/jacb.2019.67366ENT. M. S.SalimGenetic and Genetic Engineering Department, Faculty of Agriculture, Benha University, Moshtohor 13736, Qaliuobia, EgyptJournal Article20191107The point of this investigation was to decide the hereditary inconstancy and break down the connection between four faba bean cultivars and six promising lines utilizing inter simple sequence repeats (ISSR) markers. Faba bean genotypes were gathered by their resistance to the bean yellow mosaic virus infection. With the acquired band registration, a paired data matrix was built to perform the comparing statistical analysis. The used ISSR markers created 148 fixed and repeatable bands, of which 75 were polymorphic. The values of resolution power (Rp), polymorphic information content (PIC), and marker index (MI), uncovered that primer ISSR-14 was the most effective to break down hereditary changeability with estimations of 5.8, 0.80, and 6.40, respectively, followed by primers ISSR-6 and ISSR-18. Genetic distances swayed somewhere in the range of 0.78 and 0.91, and checked the groupings saw in the dendrogram, which demonstrates high change at the degree of DNA among the investigated genotypes, watching six characterized gatherings as indicated by UPGMA examination. In the examination of fundamental segments, the enrolled groupings were dictated by the source of the origin of the gathering. The utilization of ISSR markers was effective to describe at the degree of DNA the evaluated bean genotypes, showing the presence of inconstancy, the recognized differentiating promotions can be used in hereditary improvement projects planned for settling the requirements of the producers.https://jacb.journals.ekb.eg/article_67366_f3cb9d1bee417c2eb91aa1e821c439f9.pdfMansoura University, Faculty of AgricultureJournal of Agricultural Chemistry and Biotechnology2090-3626101120191101Effect of Nitrogen-Fixing Cyanobacteria on the Growth of Wheat Crop2212256736810.21608/jacb.2019.67368ENH. A. EL-ZawawyBotany Dept. (Microbiology) Fac. of Agric. Al-Azhar Univ. Cairo,, Egypt.Journal Article20191113Isolation and purification of cyanobacteria from kafr El-Sheikh soil samples revealed that, two isolates (<em>Anabaena cylindrica </em>and<em> Nostoc clacicola</em>) were acquired as bacterial free cyanobacteria and nitrogen fixed. These cynobacteria isolates were produced auxin (Indole-3-acetic acid), gibberellins (Gibberellic acid) and cytokinin (Zeatin)<em>.</em> Therefore, when inoculation with<em> Anabaena cylindrica</em>, <em>Nostoc clacicola</em> and their mixture were significantly increased the plant height, number of spikes/m<sup>2</sup>, 1000-grain weight, grain yield, straw yield protein content% on wheat crop and total counts of cyanobacteria in soil.https://jacb.journals.ekb.eg/article_67368_527051f358d0a16417c991b96e99f952.pdfMansoura University, Faculty of AgricultureJournal of Agricultural Chemistry and Biotechnology2090-3626101120191101Characterization of some Cotton Genotypes Highly Resistant to Fusarium Wilt Disease by Electrophoresis of Peroxidase Isozymes and Seed Proteins.2272306736710.21608/jacb.2019.67367ENE. M.HusseinPlant Pathology Research Institute, Agricultural Research Center, Giza, Eygpt.Marian M.HabebPlant Pathology Research Institute, Agricultural Research Center, Giza, Eygpt.Amal A.AsranPlant Pathology Research Institute, Agricultural Research Center, Giza, Eygpt.A. Z. A.AshourPlant Pathology Research Institute, Agricultural Research Center, Giza, Eygpt.A. A.AlyPlant Pathology Research Institute, Agricultural Research Center, Giza, Eygpt.Journal Article20191120Cluster analysis was used to evaluate the genetic diversity among 12 cotton genotypes highly resistant to Fusarium wilt disease. Cluster analysis was carried out based on electrophoretic banding patterns of peroxidase isozymes and seed proteins. Cluster analysis of peroxidase isozymes divided the genotypes into three distinct remotely related groups. Peroxidase isozymes were reliable to differentiate among genotypes included in the different groups; however, the isozymes were of limited reliability to differentiate among genotypes within groups. The genotypes were separated into a larg number of remotely related subclusters based on cluster analysis of their protein banding patterns. Therefore, it was easy to differentiate among the genotypes of the different subclusters. The present study demonstrated that cotton genotypes could be identified by their electrophoretic banding patterns of peroxidase isozymes and seed proteins. These results could be of practical value for cultivar identification and for seed purity tests.https://jacb.journals.ekb.eg/article_67367_ee239a6d3ada53f8c62715b90e2b5fb2.pdf