Screening for Blue-green Pigment Production by Pseudomonas Isolates

Shaaban, Dina S. M. A.; Selim, M. A. E.; Saber, W. I. A.; Elattaapy, A. M. M.

doi:10.21608/jacb.2025.435062.1127

Screening for Blue-green Pigment Production by Pseudomonas Isolates

Document Type : Original Article

Authors

¹ Microbiology Dept., Fac. of Agric., Mansoura Univ., Egypt

² Microbial Activity Unit, Microbiology Department, Soils, Water and Environment Research Institute, Agricultural Research Center, Giza 12619, Egypt

10.21608/jacb.2025.435062.1127

Abstract

Eight isolates belonging to the genus Pseudomonas were isolated from various environmental sources, including soil, water, and food material, and tested for blue-green pigment production. All selected isolates exhibited good production of the blue-green pigment, with three isolates showing high levels of pigment production, yielding 2.13, 3.47, and 5.74 µg/mL when cultured in nutrient broth medium supplemented with 1% waste oil. In an attempt to select an appropriate medium for blue-green pigment production, six different media were tested with all isolates. The Obtained data indicate a significant variation in microbial pigment production among the different culture media. The data revealed that King’s medium was the most effective for pigment production compared to nutrient broth and Bushnell Haas media with all tested Pseudomonas isolates. On the other hand, the results demonstrated that the supplementation of nutrient broth and Bushnell Haas media enhanced pigment production. Nutrient broth (Oxoid) showed high productivity with all isolates when supplemented with waste oil or glycerol. Maximum pigment production was achieved by isolate W4, yielding 4.98 µg/mL when cultured in waste oil-supplemented nutrient broth.

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