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El-Sawah, M., Kassem, M., Afify, A., Ismail, L., Menshawy, A. (2006). STUDIES ON THE PRODUCTION OF MICROBIAL B- GALACTOSIDASE 1. OPTIMIZATION OF B-GALACTOSIDASE EXTRACTION FROM Kluyveromyces fragilis AND Streptococcus salivarius SUBSP. thermophilus. Journal of Agricultural Chemistry and Biotechnology, 31(2), 999-1014. doi: 10.21608/jacb.2006.202945
M. M. A. El-Sawah; M. M. Kassem; Aida H. Afify; l. I. Ismail; Azza S. A. Menshawy. "STUDIES ON THE PRODUCTION OF MICROBIAL B- GALACTOSIDASE 1. OPTIMIZATION OF B-GALACTOSIDASE EXTRACTION FROM Kluyveromyces fragilis AND Streptococcus salivarius SUBSP. thermophilus". Journal of Agricultural Chemistry and Biotechnology, 31, 2, 2006, 999-1014. doi: 10.21608/jacb.2006.202945
El-Sawah, M., Kassem, M., Afify, A., Ismail, L., Menshawy, A. (2006). 'STUDIES ON THE PRODUCTION OF MICROBIAL B- GALACTOSIDASE 1. OPTIMIZATION OF B-GALACTOSIDASE EXTRACTION FROM Kluyveromyces fragilis AND Streptococcus salivarius SUBSP. thermophilus', Journal of Agricultural Chemistry and Biotechnology, 31(2), pp. 999-1014. doi: 10.21608/jacb.2006.202945
El-Sawah, M., Kassem, M., Afify, A., Ismail, L., Menshawy, A. STUDIES ON THE PRODUCTION OF MICROBIAL B- GALACTOSIDASE 1. OPTIMIZATION OF B-GALACTOSIDASE EXTRACTION FROM Kluyveromyces fragilis AND Streptococcus salivarius SUBSP. thermophilus. Journal of Agricultural Chemistry and Biotechnology, 2006; 31(2): 999-1014. doi: 10.21608/jacb.2006.202945

STUDIES ON THE PRODUCTION OF MICROBIAL B- GALACTOSIDASE 1. OPTIMIZATION OF B-GALACTOSIDASE EXTRACTION FROM Kluyveromyces fragilis AND Streptococcus salivarius SUBSP. thermophilus

Article 4, Volume 31, Issue 2, February 2006, Page 999-1014  XML PDF (2.03 MB)
Document Type: Original Article
DOI: 10.21608/jacb.2006.202945
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Authors
M. M. A. El-Sawah1; M. M. Kassem1; Aida H. Afify1; l. I. Ismail1; Azza S. A. Menshawy2
1Microbiol. Dept.I Fac. of Agric., Mansoura Univ.l Mansoura. Egypt.
2Fac. of Agric., Omar EI-Mokhtar Univ.. El-Bieda, Libya
Abstract
Investigation of B-galactosidase production by three yeasts and five bacterial
species of lactose-fermenting microorganisms during different incubation periods was
carried out. K. fragilis and S. salivarius subsp. thermophiius. in shaken cultures.
proved to be the most potent and produced the highest B-galactosidase activity after
24 h incubation. Toluene was the most effective soiventin releasing B-galactosidase
from the microbial cells. B-Glactosidase release was increased with the solvent
concentration till it reached its maximum activity at2.0%(vlv). Higher concentrations
were correlated with a great decrease in B-galactosidase activity. Toluene released
B-galactosidase in higher yield after 15 min. The higher contact time was correlated
with a great decrease in activity. Maximum B-galactosidase activity was extracted at
37°C during suspensions of cells in 0.1-M phosphate buffer at pH 6.8. [3-
Galactosidase release was increased with the increase of the exposure time for
sonicalion till it reached its maximum after5minutes. The maximum release off}—
galactosidase was after 6 cycles of freezing and thawing of the cell pellets and cells in
buffer. Higher activity was obtained when the pellets were used.
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