Genetic Polymorphisms of Four Egyptian Plant Species using some Molecular Markers Techniques RAPD, ISSR, and SCoT

Ahmed, Amina R.; Soliman, K. A.; Al-Senosy, Neima K.; Omar, S. A.

doi:10.21608/jacb.2022.118365.1018

Genetic Polymorphisms of Four Egyptian Plant Species using some Molecular Markers Techniques RAPD, ISSR, and SCoT

Document Type : Original Article

Authors

¹ Genetics Dept, Fac of Agric, Ain Shams Univ, P.O. Box 68, Hadayek Shoubra 11241, Cairo, Egypt.

² Cell Research Dept, Plant Ecology and Range management, Desert Rsearch Center, Ministry of Agriculture, Cairo, Egypt

10.21608/jacb.2022.118365.1018

Abstract

Four plant species belonging to the family Lamiaceae were assessed with different molecular markers using five RAPD, six inter simple sequence repeats (ISSR), and six start codon targeted (SCoT) primers to detect their levels of genetic diversity. The RAPD primers identified a total of 41 amplified bands, while all primers inducing five unique markers among the four species used in this study, furthermore the polymorphism percentage reached to 73.71%. ISSR primers generated 31 amplified bands including eight unique markers with a polymorphism percentage reached to 54.83%. SCoT primers exhibited a total of 43 amplified bands. Four of these primers revealed 14 unique genotype specific markers with a polymorphism percentage reached to 39.53%. The four Lamiaceae species were separated into two major groups using cluster analysis: the first group comprised Phlomis floccosa and Salvia officinals, while the second group included Teucrium polium and Thymus capitatus.

Keywords