PRODUCTION OF VITAMIN B12 BY Propionibacterium Freudenreichii AND Bacillus megaterium

Document Type : Original Article

Authors

Agrk. Microbial. Dept., Fac. of Agrlc., Ain Shams Unlv., P.O.Box 68, Hadayek Shoubra 11241, Cairo, Egypt.

Abstract

Effect of some nutritiona! Iactors on growth and vitamin 8'2 production by
Prop < /em>ionibacterium freudenreichii subsp. freudenreichii GeM 1857, Propionibacterium
treudenreicnii subsp. sriermenii P1NRC, Bacillus megaterium DSM 2894, and
Bacillus meqeterium 1066 was investigated. Results indicated that sodium lactate was
the best carbon source for Propionibacterium spp .. while glucose was the best carbon
source (or B. mega/anum. Yeast extract plus ammonium sulphate was superior
nitrogen source for Prop < /em>ionibacterium spp.. and yeast extract was the best nitrogen
source (or 8. megaterium. Use of combination of some compounds (GoGb ~ CuSO. ~
MnSO, ~ lnSO. + MgSO. + FeSO.) enhanced growth of Prop < /em>ionibacterium spp. and
B megaterium. Cobalt concentration of 10 mg r' was favor for Propionibacterium
spp., however cobau concentration of 15 mg r' was favor for 8. megaterium. High test
molasses and corn meal were found to be the best agriculture by-products for
Propionibacterium freudenreichii subsp. treuoerue'cm! CCM 1857 & Bacillus
megaterium DSM 2894 after being added to the basic medium.

Keywords

Journal of Agricultural Chemistry and Biotechnology
Volume 30, Issue 7
July 2005
Pages 4149-4162

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