Six pullulan producing strains of Aureobasidium pullulans were obtained and tested (or pull ulan production on three different media. All aureobasidiurn tested strains gave the maximum pullulan production on Reeselev & Jensen medium. Only one strain was selected as highly efficient pullulan producing fungus known as Aureobasidium pullufans ATCC 42023. The effect of some nutritional and environmental factors on pullulan production by this strain on Reeselev & Jensen medium were evaluated using shake flask as a batch culture. A. pullulans ATCC 42023 gave the highest pullulan production parameters after 5 days fermentation period at 2SoC in modified Reeselev & Jensen medium containing 5 % sucrose and 0.05 % glutamic acid (as a carbon and nitrogen sources, respectively) and the ratio of flask size to working volume was 2.5 ; 1. Using HCDI two-stage batch culture for pullulan production on modified Reeselev & Jensen medium (20 % sucrose) led to increase cell dry weight and puUulan productivity by 2.28 and 2.65 fold than that obtained in batch shake flasks technique.
El-Tayeb, T., Abdel Hafez, A., Abdelhady, H., & Sharaf, M. (2005). PULLULAN PRODUCTION AS AFFECTED BY Aureobasidium pullulans STRAINS AND CULTURING CONDITION.S. Journal of Agricultural Chemistry and Biotechnology, 30(7), 4163-4182. doi: 10.21608/jacb.2005.235840
MLA
T.S. El-Tayeb; A.M. Abdel Hafez; Hemmat M. Abdelhady; M.S. Sharaf. "PULLULAN PRODUCTION AS AFFECTED BY Aureobasidium pullulans STRAINS AND CULTURING CONDITION.S", Journal of Agricultural Chemistry and Biotechnology, 30, 7, 2005, 4163-4182. doi: 10.21608/jacb.2005.235840
HARVARD
El-Tayeb, T., Abdel Hafez, A., Abdelhady, H., Sharaf, M. (2005). 'PULLULAN PRODUCTION AS AFFECTED BY Aureobasidium pullulans STRAINS AND CULTURING CONDITION.S', Journal of Agricultural Chemistry and Biotechnology, 30(7), pp. 4163-4182. doi: 10.21608/jacb.2005.235840
VANCOUVER
El-Tayeb, T., Abdel Hafez, A., Abdelhady, H., Sharaf, M. PULLULAN PRODUCTION AS AFFECTED BY Aureobasidium pullulans STRAINS AND CULTURING CONDITION.S. Journal of Agricultural Chemistry and Biotechnology, 2005; 30(7): 4163-4182. doi: 10.21608/jacb.2005.235840
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