PRODUCTION OF CELLULASE ENZYMES IN A MIXED CULTURE OF Trichoderma reesel AND ',Aperg/I/us niger

Document Type : Original Article

Authors

Agrlc. Mlcroblol. Res. Dept.. Solis. Water and Environ. Res. tnst., Agrlc. Res. Center. Giza-Egypt.

Abstract

Trichoderma reesei showed higher production of CMCase (3.65 times),FPase
(3.68 times),xylanase (1.63 times), and hydrolytic power (5.4 times) than Aspergillus
niger
. However, p-glucosidase production was only about 0.135 of that of A. niger.
Mixed cultivation of the two strains Trichoderma reesei and Aspergillus niger with
modification of medium composition increased aN cenulase activities. It was 8.1 times
for CMCase. 5.9 times for FPase, 1.2 times for P-glucosidase, 3.6 times for xylanase
as compared with Aspergillus niger. The hydrolytic power (%) of the cellulase
complex produced by the mixed culture was 15.5 times in comparison with
Aspergillus niger. A comparative study was made on cellulase activities produced by
the mixed cuhure and that reported by other cellulase producers. The cellulase
enzymes production by mixed culture is comparable to that of cellulases potent
producers

Keywords