SKINETIC OF PRODUCTION OF EXOCELLULAR CATALASE BY Aspergillus phoenicis K30 MUTANT ON DATE FLOUR EXTRACT AND FUNGUS MORPHOLOGY UNDER PARTIAL OXIDATIVE STRESS

Document Type : Original Article

Authors

1 epartement des Sciences de la Nature et de la Vie, Faculte des Sciences, Universite Mentouri de Constantine 25000, Algeria

2 epartement des Sciences de la Nature et de la Vie, Faculte des Sciences, Universite Mentouri de Constantine 25000, Algeria.

3 Centre Wallon de Biologie Industrielle, Faculte Universitaire des Sciences Agronomiques, Passage des Oeportes 2, 5030 Gembloux, Belgium.

4 Fac. of Agric. Mansura Univ., Egypt

Abstract

The specific productivity of extracellular catalase by A. phoenicis K30 on
DFSE at 5408.16 U/g cell dry weights at 48 h of fermentation. This production situated
after the endocellular one. Involve that, the best total activity of enzyme (53.0 U/ml)
was produced during exponential and stationary phases of fungus growth. The
amount of the extracellular catalase activity was reflected by an increase in density
and spherical form of pellets. To that effect, in the external layer, which forms the
active zone of pellets, the ramified hypha I morphology has been showed to have an
important influence on the formation of a range of extracellular catalase by our strain.
The kinetic of fungus growth has revealed that, the stationary phase, in which, the
amount of extracellular catalase was produced was remarkably short, followed by a
rapid phase of decline. This is due, to our understanding, to the accumulation of
mycelium on metal mechanism in the tank of bioreactor. As consequence, the width of
submerged fermentation phase was considerably decreased, involved the oxidative
stress, whiCh affected directly the morphology of mycelium in submerged phase,
characterised by the cell autolysis and lessening in the exocellular catalase activity. 

Keywords

Journal of Agricultural Chemistry and Biotechnology
Volume 29, Issue 7
July 2004
Pages 4167-4177

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