Kacem -Chaouche, N., Meraihi, Z., Dehlrnat, L., Destain, J., Kacem, M., Thonart, P. (2004). SKINETIC OF PRODUCTION OF EXOCELLULAR CATALASE BY Aspergillus phoenicis K30 MUTANT ON DATE FLOUR EXTRACT AND FUNGUS MORPHOLOGY UNDER PARTIAL OXIDATIVE STRESS. Journal of Agricultural Chemistry and Biotechnology, 29(7), 4167-4177. doi: 10.21608/jacb.2004.242693
N. Kacem -Chaouche; Z. Meraihi; L. Dehlrnat; J. Destain; M. Kacem; Ph. Thonart. "SKINETIC OF PRODUCTION OF EXOCELLULAR CATALASE BY Aspergillus phoenicis K30 MUTANT ON DATE FLOUR EXTRACT AND FUNGUS MORPHOLOGY UNDER PARTIAL OXIDATIVE STRESS". Journal of Agricultural Chemistry and Biotechnology, 29, 7, 2004, 4167-4177. doi: 10.21608/jacb.2004.242693
Kacem -Chaouche, N., Meraihi, Z., Dehlrnat, L., Destain, J., Kacem, M., Thonart, P. (2004). 'SKINETIC OF PRODUCTION OF EXOCELLULAR CATALASE BY Aspergillus phoenicis K30 MUTANT ON DATE FLOUR EXTRACT AND FUNGUS MORPHOLOGY UNDER PARTIAL OXIDATIVE STRESS', Journal of Agricultural Chemistry and Biotechnology, 29(7), pp. 4167-4177. doi: 10.21608/jacb.2004.242693
Kacem -Chaouche, N., Meraihi, Z., Dehlrnat, L., Destain, J., Kacem, M., Thonart, P. SKINETIC OF PRODUCTION OF EXOCELLULAR CATALASE BY Aspergillus phoenicis K30 MUTANT ON DATE FLOUR EXTRACT AND FUNGUS MORPHOLOGY UNDER PARTIAL OXIDATIVE STRESS. Journal of Agricultural Chemistry and Biotechnology, 2004; 29(7): 4167-4177. doi: 10.21608/jacb.2004.242693
SKINETIC OF PRODUCTION OF EXOCELLULAR CATALASE BY Aspergillus phoenicis K30 MUTANT ON DATE FLOUR EXTRACT AND FUNGUS MORPHOLOGY UNDER PARTIAL OXIDATIVE STRESS
1epartement des Sciences de la Nature et de la Vie, Faculte des Sciences, Universite Mentouri de Constantine 25000, Algeria
2epartement des Sciences de la Nature et de la Vie, Faculte des Sciences, Universite Mentouri de Constantine 25000, Algeria.
3Centre Wallon de Biologie Industrielle, Faculte Universitaire des Sciences Agronomiques, Passage des Oeportes 2, 5030 Gembloux, Belgium.
4Fac. of Agric. Mansura Univ., Egypt
Abstract
The specific productivity of extracellular catalase by A. phoenicis K30 on DFSE at 5408.16 U/g cell dry weights at 48 h of fermentation. This production situated after the endocellular one. Involve that, the best total activity of enzyme (53.0 U/ml) was produced during exponential and stationary phases of fungus growth. The amount of the extracellular catalase activity was reflected by an increase in density and spherical form of pellets. To that effect, in the external layer, which forms the active zone of pellets, the ramified hypha I morphology has been showed to have an important influence on the formation of a range of extracellular catalase by our strain. The kinetic of fungus growth has revealed that, the stationary phase, in which, the amount of extracellular catalase was produced was remarkably short, followed by a rapid phase of decline. This is due, to our understanding, to the accumulation of mycelium on metal mechanism in the tank of bioreactor. As consequence, the width of submerged fermentation phase was considerably decreased, involved the oxidative stress, whiCh affected directly the morphology of mycelium in submerged phase, characterised by the cell autolysis and lessening in the exocellular catalase activity.
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