EFFECT OF AFLATOXINS ON N2-FIXER BACTERIA AND RESPONSE OF INFESTED PEANUT SEEDS WITH Aspergillus flavus TO BIOLOGICAL CONTROL

Document Type : Original Article

Authors

Soils, Water and Environment Res. Inst., Agric. Res. Center, Giza.

Abstract

UnGer lab conditions, an experiment was conducted to evaluate biological
effect of aflatoxins on diazotrophs. Also, a greenhouse experiment was executed to
study the effect of dose and time of Bacillus pumilus as a biocontrol agent application
on Aspergillus flavus infested peanut yield inoculated with Bradyrhizobium sp. Results
indicated that Azotobacter and Azosp < /em>irillum were more sensitive to aflatoxins 81, 82,
G1 and G2. However, Rhizobium and Bradyrhizobium were only affected slightly by
the aflatoxins 81 and 82 but were most resistant against G1 and G2.
Data of pots experiment showed that Bacillus pumilus caused a reduction in
Aspergillus infested peanut. This treatment decreased aflatoxin accumulation in root
zone and so enhanced nodulation, growth, yields and crude protein of peanut plants.
The reduction effect increased with the increasing of bacterial dose. The maximum
reduction of aflatoxin (62%) was obtained when the bacterial dose was 80 ml/pot.
Also, results indicated that the highest values of nodulation, growth, pod and straw
yields were obtained with Bradyrhizobium seed coating plus Bacillus p < /em>umilus when
applied at 40 ml/pot after 20 days and 30 days of planting. Data revealed that the
maximum inhibition (70%) of aflatoxin formation was obtained when two doses of
Bacillus pumilus were added (40 ml/pot of each). The first dose at planting and the
second dose after 40 days of planting.

Keywords

Journal of Agricultural Chemistry and Biotechnology
Volume 29, Issue 11
November 2004
Pages 6537-6547

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