SOLATION AND IDENTIFICATION OF SOME LOCAL ISOLATES OF Bradyrhizobium SP. (LUPINUS) USING SEROLOGICAL AND ANTIBIOTIC RESISTANCE MARKERS AND THEIR SYMBIOTIC PERFORMANCE UNDER DIFFERENT LEVELS OF N-FERTILIZATION

Document Type : Original Article

Authors

SOils, Water and Environment Res. Instit., Dept. of Soil Microbiology, ARC, Giza, Egypt.

Abstract

From eight isolates of Bradyrhizobium sp. (Lup < /em>inus) (were selected from
many isolates on the basis of their N2-fixation efficient), four different groups were
obtained and identified according to their sensitivity and resistance to antibiotics
(lAR). On the basis of serological analysis, the representative isolates of IAR groups
showed compatibility, indicating the possible grouping of indigenous isolates of lupin
rhizobia into specific groups based on serological and IAR profiles of the isolates.
Variations among indigenous rhizobial populations of the isolates from lupin was
found, and showed that there is genetic potential to improve strain performance within
rhizobial population. In order to evaluate the symbiotic performance of selected
strains, a field experiment was conducted in two locations under different levels of N-
fertilizer. The results showed that the Rhizobium inoculation and N-fertilizer had low
effect in increasing the biomass and grain yield of white lupin cultivar. No interaction
was observed between inoculation and N-fertilizer levels in all studied parameters.
The biomass and seed yield tended to be greater with Rhizobium inoculation than
with N-fertilizer. The results also indicated the potential for improvement of N2-fixation
by lupin through the application of efficient rhizobia strains such as ARC 401 and
ARC 408.

Keywords

Journal of Agricultural Chemistry and Biotechnology
Volume 29, Issue 11
November 2004
Pages 6569-6579

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