EI-Bendary,, M., Moharam, M., Foda, M. (2002). STUDIES ON PRODUCTION OF ALKALINE SERINE PROTEASE FROM Bacillus sphaericus. Journal of Agricultural Chemistry and Biotechnology, 27(2), 1231-1246. doi: 10.21608/jacb.2002.253442
Magda A. EI-Bendary,; Maysa E. Moharam; M. S. Foda. "STUDIES ON PRODUCTION OF ALKALINE SERINE PROTEASE FROM Bacillus sphaericus". Journal of Agricultural Chemistry and Biotechnology, 27, 2, 2002, 1231-1246. doi: 10.21608/jacb.2002.253442
EI-Bendary,, M., Moharam, M., Foda, M. (2002). 'STUDIES ON PRODUCTION OF ALKALINE SERINE PROTEASE FROM Bacillus sphaericus', Journal of Agricultural Chemistry and Biotechnology, 27(2), pp. 1231-1246. doi: 10.21608/jacb.2002.253442
EI-Bendary,, M., Moharam, M., Foda, M. STUDIES ON PRODUCTION OF ALKALINE SERINE PROTEASE FROM Bacillus sphaericus. Journal of Agricultural Chemistry and Biotechnology, 2002; 27(2): 1231-1246. doi: 10.21608/jacb.2002.253442
STUDIES ON PRODUCTION OF ALKALINE SERINE PROTEASE FROM Bacillus sphaericus
Microbial Chemistry Department, National Research Center, Cairo, Egypt.
Abstract
Screening studies on seventy eight strains of Bacillus sphaericus (Bs) of different origins have revealed that the majority of cultures produced extracellular alkaline protease activities in' variable levels when grown in nutrient broth- yeast extract- salt medium (NYSM) under shake culture conditions. Physiological studies on enzyme formation by two highly mosquitocidal strains namely the international strain 2362 and a local Egyptian isolate NRC 69 have shown that the enzyme was produced on a variety of different media including standard media, powdered legumes seeds broths as well as media based on agro-industrial by-products. The highest enzyme levels were obtained in both Bs cultures upon using fodder yeast, a by-product of ethanol fermentation industry, as a mono component medium for growth and ENZYME production. Maximum enzyme levels were obtained in shake cultures with medium containinJl 4% (w/v) fodder yeast under high aeration levels, inoculum size ranges 4-8 x 10 CFU/ml after three days of incubation at 30°C. The enzyme exhibited maximum activity at 55°C incubation temperature and pH 8.5 suggesting an alkaline protease activity with retention of about 60 % of the activity at pH 10 for the enzymes of both bacterial strains. Enzyme stability at 60°C was markedly improved in
the presence of calcium chloride. lnhibjticn cb~;c.:; ;;hvyycu ii,aHi~ aikanne pr~!c:::!:-:;- --~-- activity was inhibired (72% - 79%) by an active-site inhibitor of serine protease, phenyl
methylsulfonyl fluoride (PMSF) but not by soybean trypsin inhibitor or iodoacetamide ..
These results suggest that the alkaline protease activity produced belongs to serine
protease group of enzymes prevalent in some species of bacilli.
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