UULlZA TION OF SOME AGRICULTURAL BY-PRODUCTS FOR IMPROVING THE BIOSYNTHESIS OF Aspergillus ten'eus THERMOSTABLE AMYLOGLUCOSIDASE AND a· AMYLASE AND ITS USES FOR STARCH DIGESTION
The biosynthesis of Aspergillus terreus thermostable amyloglucosidase and a-amylase were improved greatly in this study, which the results revealed that:
Ingredients of the medium No. (1) used in this study gave highest enzymes productivity. Three and two days incubation improved its biosynthesis, respectively. Maltose, glucose syrup (3%), zea glutein (0.28% as nitrogen content) supported highest enzymes productivity. 2.5% (v/v) inoculum size to volume of the fermentation media, 35°C, pH 5.0 and 225 rpm as agitation rate and 1 :6.25 (vlv, vol of mediumlvol of the "ask) as aeration rate also supported highest enzymes production. PH 5.0 and 5.0 to 8.0 were found as the optimum pH for glucoamylase and a-amylase, respectively. Both enzymes were highest stable in the pH range between 4.0 to B.O. 60cC and 70°C were found as the temperature optima for both enzymes, respectively. Both enzymes were highest stable up to BO°C, indicating that, these enzymes are thermostable enzymes. K+, Na+, Mg+2 and Ca+2 strongly induced glucoamylase activity, but Fe+2 was found as slight activators of a-amylase. Hg·2 and Zn+2 inhibited both enzymes activily. Starch was found as the best substrate of these enzymes which showed highly affinity with those enzymes. Different starch substances were highly hydrolyzed with a mixture of A. taTreus glucoamylase and a-amylase, which maximum hydrolysis was attained after 5 hours of hydrolysis. This is may be due to its thermostable character and its highly affinity between enzymes and substrate. Therefore, these enzymes were highest successful in similar biotechnological process.
Shady,, T., Nasr, A., Ali, N., & Ramadan, A. (2002). UULlZA TION OF SOME AGRICULTURAL BY-PRODUCTS FOR IMPROVING THE BIOSYNTHESIS OF Aspergillus ten'eus THERMOSTABLE AMYLOGLUCOSIDASE AND a· AMYLASE AND ITS USES FOR STARCH DIGESTION. Journal of Agricultural Chemistry and Biotechnology, 27(7), 4935-4952. doi: 10.21608/jacb.2002.255927
MLA
T. S. M. Shady,; A. A. Nasr; Nadia A. A. Ali; Afaf-Hanem M. Ramadan. "UULlZA TION OF SOME AGRICULTURAL BY-PRODUCTS FOR IMPROVING THE BIOSYNTHESIS OF Aspergillus ten'eus THERMOSTABLE AMYLOGLUCOSIDASE AND a· AMYLASE AND ITS USES FOR STARCH DIGESTION", Journal of Agricultural Chemistry and Biotechnology, 27, 7, 2002, 4935-4952. doi: 10.21608/jacb.2002.255927
HARVARD
Shady,, T., Nasr, A., Ali, N., Ramadan, A. (2002). 'UULlZA TION OF SOME AGRICULTURAL BY-PRODUCTS FOR IMPROVING THE BIOSYNTHESIS OF Aspergillus ten'eus THERMOSTABLE AMYLOGLUCOSIDASE AND a· AMYLASE AND ITS USES FOR STARCH DIGESTION', Journal of Agricultural Chemistry and Biotechnology, 27(7), pp. 4935-4952. doi: 10.21608/jacb.2002.255927
VANCOUVER
Shady,, T., Nasr, A., Ali, N., Ramadan, A. UULlZA TION OF SOME AGRICULTURAL BY-PRODUCTS FOR IMPROVING THE BIOSYNTHESIS OF Aspergillus ten'eus THERMOSTABLE AMYLOGLUCOSIDASE AND a· AMYLASE AND ITS USES FOR STARCH DIGESTION. Journal of Agricultural Chemistry and Biotechnology, 2002; 27(7): 4935-4952. doi: 10.21608/jacb.2002.255927
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