THE PRODUCTION OF GIBBERELLINS FROM DIFFERENT AGRICULTURAL BY-PRODUCTS AS AFFECTED BY DIFFERENT CARBON AND NITROGEN SOURCES

Nassar,, A. R.; Abu-Shadi, A. I.; Radwan, Nabila M.

doi:10.21608/jacb.2002.256301

THE PRODUCTION OF GIBBERELLINS FROM DIFFERENT AGRICULTURAL BY-PRODUCTS AS AFFECTED BY DIFFERENT CARBON AND NITROGEN SOURCES

Document Type : Original Article

Authors

¹ Dept. of Agrfc. Blochem.; Fac. of Agric.; Ain Shams Univ., Cairo, Egypt.

² Department of Agric. Microbiol.; Soil., water and Environ. Inst., Agric. Research Center, Giza, Egypt.

10.21608/jacb.2002.256301

Abstract

Gibbere!lins production from the basal fermentation medium (synthetic liquid
medium of glucose and salts) by the fungus Gibberel/a fujikuroi NRRL 2284 was
compared with its production from sugar cane and sugar beet molasses. The
maximum production in liquid medium was 484.4 mgll after 5 days of the basal
incubation at 29 DC in shaking incubator at 200 rpm. The maximum production of
gibberellins in sugar cane molasses (200 mgll) was obtained when the sugar cane
molasses was diluted to 8% of total sugars. While in beet molasses it was 141.4 mgll
when the sugar beet molasses was diluted to 6% of total sugars. The molasses media
were supplemented with different nitrogen sources (NH.N03. NH.CI, (NH.) 2 SO.,
NH.H2PO., CH₃COONH., and corn steep liquor (CSL). The best nitrogen source was
CSL with nitrogen concentration 0.1 gil. The effect of both carbon and nitrogen
sources on the kinds of gibberellins produced was investigated by HPLC. The types of
gibberellins were affected quantitatively and qualitatively.