Nassar,, A., Abu-Shadi, A., Radwan, N. (2002). THE PRODUCTION OF GIBBERELLINS FROM DIFFERENT AGRICULTURAL BY-PRODUCTS AS AFFECTED BY DIFFERENT CARBON AND NITROGEN SOURCES. Journal of Agricultural Chemistry and Biotechnology, 27(8), 5549-5560. doi: 10.21608/jacb.2002.256301
A. R. Nassar,; A. I. Abu-Shadi; Nabila M. Radwan. "THE PRODUCTION OF GIBBERELLINS FROM DIFFERENT AGRICULTURAL BY-PRODUCTS AS AFFECTED BY DIFFERENT CARBON AND NITROGEN SOURCES". Journal of Agricultural Chemistry and Biotechnology, 27, 8, 2002, 5549-5560. doi: 10.21608/jacb.2002.256301
Nassar,, A., Abu-Shadi, A., Radwan, N. (2002). 'THE PRODUCTION OF GIBBERELLINS FROM DIFFERENT AGRICULTURAL BY-PRODUCTS AS AFFECTED BY DIFFERENT CARBON AND NITROGEN SOURCES', Journal of Agricultural Chemistry and Biotechnology, 27(8), pp. 5549-5560. doi: 10.21608/jacb.2002.256301
Nassar,, A., Abu-Shadi, A., Radwan, N. THE PRODUCTION OF GIBBERELLINS FROM DIFFERENT AGRICULTURAL BY-PRODUCTS AS AFFECTED BY DIFFERENT CARBON AND NITROGEN SOURCES. Journal of Agricultural Chemistry and Biotechnology, 2002; 27(8): 5549-5560. doi: 10.21608/jacb.2002.256301
THE PRODUCTION OF GIBBERELLINS FROM DIFFERENT AGRICULTURAL BY-PRODUCTS AS AFFECTED BY DIFFERENT CARBON AND NITROGEN SOURCES
1Dept. of Agrfc. Blochem.; Fac. of Agric.; Ain Shams Univ., Cairo, Egypt.
2Department of Agric. Microbiol.; Soil., water and Environ. Inst., Agric. Research Center, Giza, Egypt.
Abstract
Gibbere!lins production from the basal fermentation medium (synthetic liquid medium of glucose and salts) by the fungus Gibberel/a fujikuroi NRRL 2284 was compared with its production from sugar cane and sugar beet molasses. The maximum production in liquid medium was 484.4 mgll after 5 days of the basal incubation at 29 DC in shaking incubator at 200 rpm. The maximum production of gibberellins in sugar cane molasses (200 mgll) was obtained when the sugar cane molasses was diluted to 8% of total sugars. While in beet molasses it was 141.4 mgll when the sugar beet molasses was diluted to 6% of total sugars. The molasses media were supplemented with different nitrogen sources (NH.N03. NH.CI, (NH.) 2 SO., NH.H2PO., CH3COONH., and corn steep liquor (CSL). The best nitrogen source was CSL with nitrogen concentration 0.1 gil. The effect of both carbon and nitrogen sources on the kinds of gibberellins produced was investigated by HPLC. The types of gibberellins were affected quantitatively and qualitatively.
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