EFFECT OF Azotobacter chroococcum AND Azospirillum brasilense INOCULATION AND ANHYDROUS AMMONIA ON ROOT COLONIZATION, PLANT GROWTH AND YIELD OF WHEAT PLANT UNDER SALINE ALKALINE CONDITIONS

Document Type : Original Article

Authors

1 Agric. Microblol. Res. Dept., Soil, Water and Environ. Res. Institute, Agrlc. Res. Center, Giza, Egypt.

2 Plant Nutrition Res. Dept., Soil. Water and Environ. Res. Institute. Agrlc. Res. Center, Giza, Egypt.

Abstract

A field experiment was conducted to study the effect of different rates of
nitrogen (anhydrous ammonia at O. 20, 40. 60 and 80 Kg N fed-I) and single or dual
inoculation with Azosp < /em>irillum brasi/ense and Azotobacter chroococcum on plant
growth and N-uptake of wheat at Abo Eash (Fayoum Governorate) during the growing
season of 2000-2001. The experimental design was split-split plot in a randomized
complete biocks with three replicates. The obtained data showed significant
increases in dry weight and yield either by increasing the rate of nitrogen application
or with dual inoculation. All the inoculants gave significantly higher grain and straw
yield and nitrogen uptake by wheat plant than other uninoculated treatment. it was
observed that dual inoculation with Azotobacter chroococcum and Azospirillum
brasilense perfonned significantly better followed by single inoculation with
Azotobacter or Azospirillum. The maximum benefit was obtained with combined
inoculation at all levels of N-fertilizer and increased all values of wheat yield and its
components. The results illustrated that, incremental rates of applied nitrogenous
fertilizer to wheat plants gradually increased the means of N-uptake by both grains
and straw. It could be concluded that a substantial amount of chemical N fertilizer
could be saved by using biofertilizer which In turn minimizes the production costs and
pollution factors, which can occur by the excess use of chemical fertilizers.

Keywords

Journal of Agricultural Chemistry and Biotechnology
Volume 27, Issue 8
August 2002
Pages 5575-5591

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