Home Articles List Article Information
Journal of Agricultural Chemistry and Biotechnology
Journal Archive
Volume Volume 16 (2025)
Volume Volume 15 (2024)
Volume Volume 14 (2023)
Volume Volume 13 (2022)
Volume Volume 12 (2021)
Volume Volume 11 (2020)
Volume Volume 10 (2019)
Volume Volume 9 (2018)
Volume Volume 8 (2017)
Volume Volume 7 (2016)
Volume Volume 6 (2015)
Volume Volume 5 (2014)
Volume Volume 4 (2013)
Volume Volume 3 (2012)
Volume Volume 2 (2011)
Volume Volume 1 (2010)
Volume Volume 34 (2009)
Volume Volume 33 (2008)
Volume Volume 32 (2007)
Volume Volume 31 (2006)
Volume Volume 30 (2005)
Volume Volume 29 (2004)
Volume Volume 28 (2003)
Volume Volume 27 (2002)
Volume Volume 26 (2001)
Volume Volume 25 (2000)
El-Hawary,, F., Afify, A., Ismail, I., EI-Shafey, A. (2002). PURIFICATION AND IMMOBILIZATION OF LIPASE PRODUCED FROM Pseudomonas aeruginosa. Journal of Agricultural Chemistry and Biotechnology, 27(9), 6381-6392. doi: 10.21608/jacb.2002.257015
Fatma I. El-Hawary,; Aida H. Afify; I. I. Ismail; Amal Y. M. I. EI-Shafey. "PURIFICATION AND IMMOBILIZATION OF LIPASE PRODUCED FROM Pseudomonas aeruginosa". Journal of Agricultural Chemistry and Biotechnology, 27, 9, 2002, 6381-6392. doi: 10.21608/jacb.2002.257015
El-Hawary,, F., Afify, A., Ismail, I., EI-Shafey, A. (2002). 'PURIFICATION AND IMMOBILIZATION OF LIPASE PRODUCED FROM Pseudomonas aeruginosa', Journal of Agricultural Chemistry and Biotechnology, 27(9), pp. 6381-6392. doi: 10.21608/jacb.2002.257015
El-Hawary,, F., Afify, A., Ismail, I., EI-Shafey, A. PURIFICATION AND IMMOBILIZATION OF LIPASE PRODUCED FROM Pseudomonas aeruginosa. Journal of Agricultural Chemistry and Biotechnology, 2002; 27(9): 6381-6392. doi: 10.21608/jacb.2002.257015

PURIFICATION AND IMMOBILIZATION OF LIPASE PRODUCED FROM Pseudomonas aeruginosa

Article 2, Volume 27, Issue 9, September 2002, Page 6381-6392  XML PDF (876.32 K)
Document Type: Original Article
DOI: 10.21608/jacb.2002.257015
View on SCiNiTO View on SCiNiTO
Authors
Fatma I. El-Hawary,; Aida H. Afify; I. I. Ismail; Amal Y. M. I. EI-Shafey
Dept. of Microbiology, Fac. of Agric., Mansoura Univ., Mansoura, Egypt
Abstract
The lipases of P. aeruginosa were isolated, immobilized and characterized.
They were applied to hydrolysis of crude oils i.e. cotton oil, maize oil, palm oil,
sunflower oil and olive oil. The lipase produced by P. aeruginos8 was precipitated
from the growth media using by cold acetone and was immobilized on celite. The
characteristics of free and immobilized lipases were studied as follows:
The optimum ternperature for Iipases activity was 37°C for free lipase.
whereas it was 44°C for immobilized one. The optimum pH value for free and
Immobilized lipases was 7.4. The stability to pH was higher in Ihe Immobifized
enzyme than the free one. Immobilized lipase was stable between pH 6.8-7.8 and
tree lipase between 7.2-7.6. Both free and immobilized lipases were stable at 40·C
but immobilized one was more stable at (4'J-SO°C). The optimum substrate
concentrations for free and immobilized Iipases were 0.25% and 0.30"/0, respectively.
Free and immobilized lipases were able 10 hydrolyse all natural oils tested. Olive olf
was the most hydrolyzed one than other oils.
Keywords
Immobilized lipase; oils. Pseudomonas aeruginosa
Statistics
Article View: 91
PDF Download: 237