Biological control of two pathogenic fungi by silicate bacteria

Document Type : Original Article

Authors

1 Department of Agricultural Microbiology, Faculty of Agriculture, Mansoura University, Mansoura, Egypt

2 Plant Pathology Research Institute, Agriculture Research Center (ARC), Giza, Egypt

Abstract

Silicate solubilizing bacteria (SSB) play a critical role in soil fertility by utilizing available forms of silicates minerals. Furthermore, SSB can also enhance plant defense mechanisms. Distribution of rhizobacteria in fields had been cultivated with flax and wheat which, are high silicate accumulators, and screened for available silicate on a selective medium, the bacterial counts varied markedly with a type of plantation in soil. Four bacterial strains were capable of solubilizing biotite (source of silicate mineral): Pseudomonas fluorescens, Bacillus subtilis, Proteus mirabilis, and Bacillus circulans. By using dual culture antagonistic assays were carried out on these bacteria versus two pathogens fungi (Fusarium oxysporum and Rhizoctonia solani). The inhibition zone of these bacteria against two pathogenic fungi ranged 10 to 33 mm. The maximum inhibition zone by bacterial strains was shown by the strain B. subtilis followed by Ps. fluorescens. Whendetected the antagonistic metabolites, siderophores were produced by Ps. fluorescens and B. subtilis also these two strains with B. criculans produced chitinase. Only Proteus mirabilis produced hydrogen cyanide. The SSB strains showed different levels of efficiency in increasing the survival of flax seedlings however, two bacterial strains (B. subtilis & Ps. fluorescens) showed maximum efficiency under glasshouse conditions compared with the control. Finally, the strong action of silicate bacteria is very important in the development of bio fungicides against fungal pathogens

Keywords

Journal of Agricultural Chemistry and Biotechnology
Volume 13, Issue 10
October 2022
Pages 91-94

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