Home Articles List Article Information
Journal of Agricultural Chemistry and Biotechnology
Journal Archive
Volume Volume 15 (2024)
Volume Volume 14 (2023)
Volume Volume 13 (2022)
Volume Volume 12 (2021)
Volume Volume 11 (2020)
Volume Volume 10 (2019)
Volume Volume 9 (2018)
Volume Volume 8 (2017)
Volume Volume 7 (2016)
Volume Volume 6 (2015)
Volume Volume 5 (2014)
Volume Volume 4 (2013)
Volume Volume 3 (2012)
Volume Volume 2 (2011)
Volume Volume 1 (2010)
Volume Volume 34 (2009)
Volume Volume 33 (2008)
Volume Volume 32 (2007)
Volume Volume 31 (2006)
Volume Volume 30 (2005)
Volume Volume 29 (2004)
Volume Volume 28 (2003)
Volume Volume 27 (2002)
Volume Volume 26 (2001)
Volume Volume 25 (2000)
Hasaneen, M., El-Sayed, A., Sabry, S. (2018). Identification, Characterization and Optimized Antimicrobial Production of Streptomyces thinghirensis Isolate. Journal of Agricultural Chemistry and Biotechnology, 9(12), 263-268. doi: 10.21608/jacb.2018.36315
M. Hasaneen; A. El-Sayed; Sara Sabry. "Identification, Characterization and Optimized Antimicrobial Production of Streptomyces thinghirensis Isolate". Journal of Agricultural Chemistry and Biotechnology, 9, 12, 2018, 263-268. doi: 10.21608/jacb.2018.36315
Hasaneen, M., El-Sayed, A., Sabry, S. (2018). 'Identification, Characterization and Optimized Antimicrobial Production of Streptomyces thinghirensis Isolate', Journal of Agricultural Chemistry and Biotechnology, 9(12), pp. 263-268. doi: 10.21608/jacb.2018.36315
Hasaneen, M., El-Sayed, A., Sabry, S. Identification, Characterization and Optimized Antimicrobial Production of Streptomyces thinghirensis Isolate. Journal of Agricultural Chemistry and Biotechnology, 2018; 9(12): 263-268. doi: 10.21608/jacb.2018.36315

Identification, Characterization and Optimized Antimicrobial Production of Streptomyces thinghirensis Isolate

Article 1, Volume 9, Issue 12, December 2018, Page 263-268  XML PDF (999.05 K)
Document Type: Original Article
DOI: 10.21608/jacb.2018.36315
View on SCiNiTO View on SCiNiTO
Authors
M. Hasaneen1; A. El-Sayed2; Sara Sabry2
1Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt
2Botany and Microbiology Department, Faculty of Science, Damietta University, Damietta El-Gededa, PO 34517, Egypt
Abstract
The present study was conducted to isolate, identify and characterize the Streptomyces strain using morphological and biochemical analysis. This strain was molecular identified by sequencing the 16S rRNA gene as Streptomyces thinghirensis EGDA6S isolate. Results for scanning electron microscopy (SEM) revealed that the tested isolate is belonging to yellow series of Streptomycetes with smooth spore surface. Its cell wall contains LL-Diaminopimelic acid (LL-DAP) with non-characteristic sugar pattern and it does not produce melanin pigmentation. S. thinghirensis EGDA6S' metabolite revealed a considrable antimicrobial activity against some microorganisms namely: Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. Whereas, the maximum antimicrobial activity was observed on oat meal followed by starch nitrate media. Maximum antimicrobial activity being 7 days, at 30oC and pH 7, for optimum incubation period, temperature and pH.
Keywords
Streptomyces; Optimization; 16S rDNA sequencing; antimicrobial activity
Statistics
Article View: 273
PDF Download: 654