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El-Fadaly, H., El Kadi, S., El-Rian, M. (2019). Isolation, Characterization and Identification of Salt Tolerant Nitrogen Fixing Bacteria. Journal of Agricultural Chemistry and Biotechnology, 10(4), 73-78. doi: 10.21608/jacb.2019.37016
H. El-Fadaly; S. El Kadi; M. El-Rian. "Isolation, Characterization and Identification of Salt Tolerant Nitrogen Fixing Bacteria". Journal of Agricultural Chemistry and Biotechnology, 10, 4, 2019, 73-78. doi: 10.21608/jacb.2019.37016
El-Fadaly, H., El Kadi, S., El-Rian, M. (2019). 'Isolation, Characterization and Identification of Salt Tolerant Nitrogen Fixing Bacteria', Journal of Agricultural Chemistry and Biotechnology, 10(4), pp. 73-78. doi: 10.21608/jacb.2019.37016
El-Fadaly, H., El Kadi, S., El-Rian, M. Isolation, Characterization and Identification of Salt Tolerant Nitrogen Fixing Bacteria. Journal of Agricultural Chemistry and Biotechnology, 2019; 10(4): 73-78. doi: 10.21608/jacb.2019.37016

Isolation, Characterization and Identification of Salt Tolerant Nitrogen Fixing Bacteria

Article 1, Volume 10, Issue 4, April 2019, Page 73-78  XML PDF (859.09 K)
Document Type: Original Article
DOI: 10.21608/jacb.2019.37016
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Authors
H. El-Fadaly; S. El Kadi; M. El-Rian
Agriculture microbiology Department, Faculty of Agricultural, Damietta University, Damietta Egypt
Abstract
The present investigation deals with the isolation and characterization of nitrogen-fixing bacteria (NFB) such as Azotobacter sp. and Azospirillum sp. from paddy field rhizosphere of Damietta governorate, Egypt. Twelve Azotobacter spp. and 12 Azospirillum spp.Were identified based on morphological and physiological characteristics. All isolates were tested for salt tolerance. The highest salt tolerant of Azotobacter spp. Were S2-8, S3-3, S1-3, S3-2, whil they S2-11, S1-1, S5-1, S3-6 in case of Azospirillum spp. were. Nitrogenase activity assay of the highest nitrogen fixing isolates was (S2-8) in case of Azotobacter sp. and (S2-11)in case of Azospirillum sp. Molecular identification showed that isolates were Azotobacter chroococcum and Azospirillm brasilense. Based on this study salt-tolerant isolates of Azotobacter chroococcum and Azospirillm brasilense could be highly used in cultivation of paddy soil in coastal environment.
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