Bio-hydrogen Production by Escherichia coli NRRL B-3008 and Pseudomonas aeruginosa ATCC 27853 Using Microbial Electrolysis Cells

Document Type : Original Article

Authors

1 Microbiology Dept., Fac. Agric., Mansoura Univ., Mansoura, Egypt.

2 Soil Fertility and Microbiology Dept., Desert Res. Center, Matariya, Cairo, Egypt.

Abstract

Industrial wastewater was used as the substrate for bio-hydrogen ( Bio-H2 ) production in Microbial Electrolysis Cells
(MECs) by Escherichia coli NRRL B-3008 and Pseudomonas aeruginosa  ATCC 27853. Three volumes of anode chamber in MECs 300ml, 400 ml and 500 ml were applied. Salt bridge was used for the exchange of protons from anode to cathode chamber. External voltage of 0.4 V, 0.6 V and 0.8 V was used applied to  MECs using a regulated power supply. The highest volume of Bio-H2  358.24 cm3  and 343.57cm3 at the anode chamber 500 ml with power supply 0.8 V  by Escherichia coli NRRL B-3008 and Pseudomonas aeruginosa  ATCC 27853 respectively .

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