Abdelhadi, A., Mohamed, M. (2016). Antibacterial and Mutagenic Potency Screening of Different Pomegranate Cultivars Using rep-PCR. Journal of Agricultural Chemistry and Biotechnology, 7(12), 297-301. doi: 10.21608/jacb.2016.41149
A. Abdelhadi; M. Mohamed. "Antibacterial and Mutagenic Potency Screening of Different Pomegranate Cultivars Using rep-PCR". Journal of Agricultural Chemistry and Biotechnology, 7, 12, 2016, 297-301. doi: 10.21608/jacb.2016.41149
Abdelhadi, A., Mohamed, M. (2016). 'Antibacterial and Mutagenic Potency Screening of Different Pomegranate Cultivars Using rep-PCR', Journal of Agricultural Chemistry and Biotechnology, 7(12), pp. 297-301. doi: 10.21608/jacb.2016.41149
Abdelhadi, A., Mohamed, M. Antibacterial and Mutagenic Potency Screening of Different Pomegranate Cultivars Using rep-PCR. Journal of Agricultural Chemistry and Biotechnology, 2016; 7(12): 297-301. doi: 10.21608/jacb.2016.41149
Antibacterial and Mutagenic Potency Screening of Different Pomegranate Cultivars Using rep-PCR
1Genetics Department, Faculty of Agriculture, Cairo University, Egypt
2Botany and Microbiology Department, Faculty of Sciences, Cairo University, Egypt
Abstract
Pomegranate fruit is a rich source of antimicrobial agents. Herein, the antibacterial potencies of four pomegranate cultivar peel extracts; Wonderful, H116, Manfalouty and Black, against five pathogenic bacteria Staphylococcus aureus,Bacillus cereus, Salmonella typhimurium, Pseudomonas aeruginosa and Escherichia coli have been evaluated with routine disc diffusion susceptibility testing. The gross effects of peel extracts on bacterial DNA have been assessed with the rep-PCR DNA fingerprinting technique. The results indicated that all cultivar peel extracts showed enhanced antibacterial activities against the tested strains compared to controls. The polymorphic DNA banding patterns of E. coli O157:H7, as a reference strain, treated with different cultivar peel extracts were used as a reference test to detect the mutagenic effect of peel extracts. The study indicated that pomegranate peel is a promising source as antibacterial agents or bacterial mutagens.
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