Ali, S. (2025). Plackett-Burman Design of Low-Cost Culture Medium for Biomass Production of Probiotic Bacillus licheniformis CCASU-2024-65 and Bacillus subtilis (CCASU-2024-67) Strains. Journal of Agricultural Chemistry and Biotechnology, 16(2), 21-26. doi: 10.21608/jacb.2025.356888.1102
Shimaa K. Ali. "Plackett-Burman Design of Low-Cost Culture Medium for Biomass Production of Probiotic Bacillus licheniformis CCASU-2024-65 and Bacillus subtilis (CCASU-2024-67) Strains". Journal of Agricultural Chemistry and Biotechnology, 16, 2, 2025, 21-26. doi: 10.21608/jacb.2025.356888.1102
Ali, S. (2025). 'Plackett-Burman Design of Low-Cost Culture Medium for Biomass Production of Probiotic Bacillus licheniformis CCASU-2024-65 and Bacillus subtilis (CCASU-2024-67) Strains', Journal of Agricultural Chemistry and Biotechnology, 16(2), pp. 21-26. doi: 10.21608/jacb.2025.356888.1102
Ali, S. Plackett-Burman Design of Low-Cost Culture Medium for Biomass Production of Probiotic Bacillus licheniformis CCASU-2024-65 and Bacillus subtilis (CCASU-2024-67) Strains. Journal of Agricultural Chemistry and Biotechnology, 2025; 16(2): 21-26. doi: 10.21608/jacb.2025.356888.1102
Plackett-Burman Design of Low-Cost Culture Medium for Biomass Production of Probiotic Bacillus licheniformis CCASU-2024-65 and Bacillus subtilis (CCASU-2024-67) Strains
Microbiology Department, Faculty of Agriculture, Beni-Suef University, 62511, Egypt.
Abstract
The management of agro-waste and its conversion into useable products using biotechnological applications in agriculture is garnering significant interest. Bacillus strains are commonly used in poultry cultures as probiotics or single-cell proteins. However, their high production costs and low biomass yields limit their use as microbial proteins in animal feeds. Biomass production of Bacillus licheniformis and Bacillus subtilis is a growing area of interest in agriculture due to its nutritional benefits. This study evaluated various agro-waste culture media for biomass production of these probiotics, utilizing banana, pea, rice husks, and potato peels. The highest biomass production was achieved by Bacillus licheniformis (CCASU-2024-65) at 1.97 g/L with banana peel extract medium, while Bacillus subtilis (CCASU-2024-67) recorded high biomass production of 1.5 g/L with potato peel extract medium. The statistical Plackett-Burman Design was used to screen nutrient components and culture conditions for these probiotics. The maximum biomass production of Bacillus licheniformis was 2.7 g/l, achieved at run number 8, which had ideal conditions comprising an initial pH of 6, 50 g/L of banana peel extract, 50 g/L of pea peel extract, a 10% inoculation ratio, and a fermentation duration of 48 hours for biomass generation. Bacillus subtilis produced 3.52 g/l output at run number 12, which had output occurred at run number 12, which comprised 50 g/L potato peel extract and 50 g/L pea peel extract concentration, an initial pH of 7, a 10% inoculum size, and a fermentation duration of 48 hours.
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