Production of Cellulases Via Solid State Fermentation by Different Fungal Isolates using Various Agricultural Wastes as a Substrate

Document Type : Original Article

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Microbiology Dept., Fac. of Agric., Mansoura Univ.

Abstract

Four local cellulolytic fungal isolates were chosen among 30 isolates according to CMC-ase production via SSF using clover straw as agricultural waste. Isolates were identified as Trichoderma asperellum, Aspergillus flavus, Aspergillus flavipes, Aspergillus terreus. Different agricultural wastes were used for CMC-ase production by the isolates and the combination of clover straw and wheat brane (1:1) proved to be the best for CMC-ase production by the four isolates, while low values of CMC-ase were noticed by all fungal isolates by using the other agricultural wastes. Molasses proved to be the best additional carbon source for CMC-ase production by all tested fungi, also cellulose and carboxymethyl cellulose enhanced CMC-ase production. All addition nitrogen sources improved CMC-ase production, yeast extract and ammonium sulphate were the best nitrogen sources for CMC-ase production by Aspergillus flavus and Trichoderma asperellum, while ammonium chloride proved to be the best nitrogen source for CMC-ase production by Aspergillus flavipes and Aspergillus terreus. Trichoderma asperellum is in the first order in CMC-ase production followed by Aspergillus flavus, Aspergillus flavipes and Aspergillus terreus. The Three isolates of Aspergillus flavus, Aspergillus terreus and Aspergillus flavipes proved to be good producers for FP-ase, while few amount of FP-ase is produced by Trichoderma asperellum.

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