Abou –Taleb, K., Mashhoor, W., Nasr, S., Sharaf, M. (2015). NUTRITIONAL AND ENVIRONMENTAL FACTORS AFFECTING CELLULASES ACTIVITY PRODUCED BY HIGH POTENT CELLULOLYTIC BACILLI. Journal of Agricultural Chemistry and Biotechnology, 6(2), 29-39. doi: 10.21608/jacb.2015.44030
Khadiga Abou –Taleb; W. Mashhoor; Sohair Nasr; M. Sharaf. "NUTRITIONAL AND ENVIRONMENTAL FACTORS AFFECTING CELLULASES ACTIVITY PRODUCED BY HIGH POTENT CELLULOLYTIC BACILLI". Journal of Agricultural Chemistry and Biotechnology, 6, 2, 2015, 29-39. doi: 10.21608/jacb.2015.44030
Abou –Taleb, K., Mashhoor, W., Nasr, S., Sharaf, M. (2015). 'NUTRITIONAL AND ENVIRONMENTAL FACTORS AFFECTING CELLULASES ACTIVITY PRODUCED BY HIGH POTENT CELLULOLYTIC BACILLI', Journal of Agricultural Chemistry and Biotechnology, 6(2), pp. 29-39. doi: 10.21608/jacb.2015.44030
Abou –Taleb, K., Mashhoor, W., Nasr, S., Sharaf, M. NUTRITIONAL AND ENVIRONMENTAL FACTORS AFFECTING CELLULASES ACTIVITY PRODUCED BY HIGH POTENT CELLULOLYTIC BACILLI. Journal of Agricultural Chemistry and Biotechnology, 2015; 6(2): 29-39. doi: 10.21608/jacb.2015.44030
NUTRITIONAL AND ENVIRONMENTAL FACTORS AFFECTING CELLULASES ACTIVITY PRODUCED BY HIGH POTENT CELLULOLYTIC BACILLI
Agric. Microbiol. Dept., Fac. of Agric., Ain Shams Univ., P.O.Box 68, Hadayek Shoubra 11241, Cairo, Egypt.
Abstract
Effect of some nutritional and environmental factors on growth and cellulase production by Bacillus alcalophilus S39 andBacillusamyloliquefaciens C23 was investigated. Results indicated that 1% carboxymethylcellulose (CMC) and 0.7% yeast extract were most effective as the carbon and nitrogen sources respectively. Initial pH 7 and 3% inoculum size (3.5-4.3 ×106 CFU/ml) found to be optimal for growth and cellulase production. Incubation temperature at 30ºC and 45ºC achieved the highest activity of cellulase for Bacillus alcalophilus S39and Bacillusamyloliquefaciens C23 respectively, and the suitable shaking rate was 150 and 200 rpm.
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