Eldenary, M. (2015). SEMI QUANTITATIVE RT PCR ANALYSIS OF TWO SILICON TRANSPORTER GENES IN SILICON UPTAKE AND STEM BORER RESISTANCE IN SOME RICE GENOTYPES. Journal of Agricultural Chemistry and Biotechnology, 6(8), 285-292. doi: 10.21608/jacb.2015.48413
M. E. Eldenary. "SEMI QUANTITATIVE RT PCR ANALYSIS OF TWO SILICON TRANSPORTER GENES IN SILICON UPTAKE AND STEM BORER RESISTANCE IN SOME RICE GENOTYPES". Journal of Agricultural Chemistry and Biotechnology, 6, 8, 2015, 285-292. doi: 10.21608/jacb.2015.48413
Eldenary, M. (2015). 'SEMI QUANTITATIVE RT PCR ANALYSIS OF TWO SILICON TRANSPORTER GENES IN SILICON UPTAKE AND STEM BORER RESISTANCE IN SOME RICE GENOTYPES', Journal of Agricultural Chemistry and Biotechnology, 6(8), pp. 285-292. doi: 10.21608/jacb.2015.48413
Eldenary, M. SEMI QUANTITATIVE RT PCR ANALYSIS OF TWO SILICON TRANSPORTER GENES IN SILICON UPTAKE AND STEM BORER RESISTANCE IN SOME RICE GENOTYPES. Journal of Agricultural Chemistry and Biotechnology, 2015; 6(8): 285-292. doi: 10.21608/jacb.2015.48413
SEMI QUANTITATIVE RT PCR ANALYSIS OF TWO SILICON TRANSPORTER GENES IN SILICON UPTAKE AND STEM BORER RESISTANCE IN SOME RICE GENOTYPES
Department of Genetics, Faculty of Agriculture,Tanta University, Egypt.
Abstract
Relative gene expression of two Si transporter genes (Lsi1 and Lsi2) was examined in the roots of seven tested rice genotypes grown in solution culture (9 days old) using semi‑quantitative RT‑PCR technique. The Si concentration in roots, stems and shoots of these rice accessions (30 days old grown in solution culture) was measured. Moreover, stem diameter, white head percentage and plant Si content were studied at the heading stage for the tested rice genotypes grown in field in order to insure stem borer resistance trait in the higher Si content and lower stem diameter rice plants. The results of this study indicate the direct association of the level of gene expression of Si transporter genes and plant Si content and its associated characters with stem borer resistance trait in rice.
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