Afify, A., Hauka, F., Kassem, M., El-Sawah, A. (2015). ENHANCEMENT OF INULINASE PRODUCTION FROM AN Acetobacter sp. IN SUBMERGED CULTURES. Journal of Agricultural Chemistry and Biotechnology, 6(10), 435-445. doi: 10.21608/jacb.2015.48443
Aida H. Afify; F.I.A. Hauka; M.M. Kassem; A.M. El-Sawah. "ENHANCEMENT OF INULINASE PRODUCTION FROM AN Acetobacter sp. IN SUBMERGED CULTURES". Journal of Agricultural Chemistry and Biotechnology, 6, 10, 2015, 435-445. doi: 10.21608/jacb.2015.48443
Afify, A., Hauka, F., Kassem, M., El-Sawah, A. (2015). 'ENHANCEMENT OF INULINASE PRODUCTION FROM AN Acetobacter sp. IN SUBMERGED CULTURES', Journal of Agricultural Chemistry and Biotechnology, 6(10), pp. 435-445. doi: 10.21608/jacb.2015.48443
Afify, A., Hauka, F., Kassem, M., El-Sawah, A. ENHANCEMENT OF INULINASE PRODUCTION FROM AN Acetobacter sp. IN SUBMERGED CULTURES. Journal of Agricultural Chemistry and Biotechnology, 2015; 6(10): 435-445. doi: 10.21608/jacb.2015.48443
ENHANCEMENT OF INULINASE PRODUCTION FROM AN Acetobacter sp. IN SUBMERGED CULTURES
Recently, inulinases one of the most important enzymes which have gained much attention in industrial purposes. They can be applied for production of high fructose syrup from inulin and could reduce the process steps, resulting in cost reduction and increasing the amount of products. Acetobacter sp. produced extracellular inulinase in vitro. Maximum inulinase production was obtained at pH 7.0, 300C temperature, ammonium sulphate as nitrogen source, inoculum volume 5% (v/v), 1% sucrose as additional carbon source and 48 hr after inoculation.The enzyme showed maximum activity at pH 7 and 50 0C. The enzyme was stable more than 80% of its maximal activity at 30-50 0C after 30 min heat treatment.