PROTECTIVE EFFECTS OF GRAPE SEED OIL AGAINST CCl4 INDUCED OXIDATIVE STRESS IN RAT BRAIN

Ismail, A.F.M.; Eassawy, M.M.T.; Salem, A.A.M.

doi:10.21608/jacb.2014.50047

PROTECTIVE EFFECTS OF GRAPE SEED OIL AGAINST CCl4 INDUCED OXIDATIVE STRESS IN RAT BRAIN

Document Type : Original Article

Authors

¹ Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT).Nasr City, Cairo, Egypt.

² Regional Center for Food and Feed (RCFF), Agriculture Research Center, Giza, Egypt.

10.21608/jacb.2014.50047

Abstract

This study aimed to investigate the possible beneficial effects of grape seed oil (GSO) on CCl₄-induced acute brain toxicity in rats. The animals injected with acute dose of CCl₄ (2 ml / kg b. wt.) showed a statistical significant decrease in the blood hematological parameters (WBCs, RBCs, PLT counts, Hb and MCV values), and a significant elevation in serum TNF-α, and IL-6 levels. In addition, damage of the brain DNA, disturbance of the brain antioxidant status; a significant decrease in SOD, GSH-Px and Catalase (CAT) activities as well as GSH level, accompanied with a significant elevation in MDA and NO levels, as well as a high significant elevation in xanthine oxidase and inducible nitric oxide synthase (iNOS) gene expressions have been observed due to the oxidative stress produced after CCl₄ injection. The pretreatment of GSO exert significant ameliorated the hematologic parameters, and serum TNF-α and IL-6 levels, protected DNA damage, improved SOD, GSH-Px and CAT activities as well as GSH, MDA and NO levels and down-regulation of xanthine oxidase (XO) and inducible nitric oxide synthase (iNOS)gene expression levels in the brain tissues of CCl₄ injected rats. These findings suggest that GSO prevents acute brain damage due to CCl₄ toxicity, which could be attributed to its immuno-modulation and antithrombotic, antiapototic, antioxidant, and anti-inflammatory activities. It can be suggested that GSO which containing high level of polyphenolic compounds, essential fatty acids and vitamin E (Tocopherol) revealed to protect the brain from CCl₄ toxicity and/or any other toxicant cause oxidative stress.

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