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El - Adl, A., Kash, K., Zaied, K., Kamal, M. (2013). EFFECT OF ENDOXAN AND ETHYLE METHANE SULPHONATE ON CHROMOSOMAL BEHAVIOUR AND MITOTIC INDEX IN SOMATIC CELLS OF ONION ( Allium cepa ,L .). Journal of Agricultural Chemistry and Biotechnology, 4(3), 93-102. doi: 10.21608/jacb.2013.53027
A. M. El - Adl; Kawther S. Kash; K. A. Zaied; Mervat I. Kamal. "EFFECT OF ENDOXAN AND ETHYLE METHANE SULPHONATE ON CHROMOSOMAL BEHAVIOUR AND MITOTIC INDEX IN SOMATIC CELLS OF ONION ( Allium cepa ,L .)". Journal of Agricultural Chemistry and Biotechnology, 4, 3, 2013, 93-102. doi: 10.21608/jacb.2013.53027
El - Adl, A., Kash, K., Zaied, K., Kamal, M. (2013). 'EFFECT OF ENDOXAN AND ETHYLE METHANE SULPHONATE ON CHROMOSOMAL BEHAVIOUR AND MITOTIC INDEX IN SOMATIC CELLS OF ONION ( Allium cepa ,L .)', Journal of Agricultural Chemistry and Biotechnology, 4(3), pp. 93-102. doi: 10.21608/jacb.2013.53027
El - Adl, A., Kash, K., Zaied, K., Kamal, M. EFFECT OF ENDOXAN AND ETHYLE METHANE SULPHONATE ON CHROMOSOMAL BEHAVIOUR AND MITOTIC INDEX IN SOMATIC CELLS OF ONION ( Allium cepa ,L .). Journal of Agricultural Chemistry and Biotechnology, 2013; 4(3): 93-102. doi: 10.21608/jacb.2013.53027

EFFECT OF ENDOXAN AND ETHYLE METHANE SULPHONATE ON CHROMOSOMAL BEHAVIOUR AND MITOTIC INDEX IN SOMATIC CELLS OF ONION ( Allium cepa ,L .)

Article 1, Volume 4, Issue 3, March 2013, Page 93-102  XML PDF (606.45 K)
Document Type: Original Article
DOI: 10.21608/jacb.2013.53027
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Authors
A. M. El - Adl; Kawther S. Kash; K. A. Zaied; Mervat I. Kamal
Dept. of Genetics , Fac. of Agric. , Mans. Univ. , Egypt.
Abstract
This study aimed to investigate abnormal chromosomal behaviour in mitosis resulted from Ethyle methane sulphonate ( EMS ) and Endoxan . Commercial onion bulbs were used as a material of  mitotic  cell division  . In addition , this study compared  between the effect of mutagenic agent ( EMS ) with anticancer compound  (Endoxan ) on mitotic index  . Onion bulbs were germinated under the effect of EMS and Endoxan , in addition to control treated with  tap water . The differences between treatments and  control were statistically evaluated . The results achieved that mitotic index of control had the largest number of normally divided cells  about 15% , while Endoxan  about 10% and EMS about 4% . This indicated  that EMS had a strong effect of inhibiting mitosis than of Endoxan.    In general , Endoxan and EMS reduced mitotic index than control  in addition to several types of chromosomal aberrations . These chemical agents included sticky chromosomes , anaphase bridge chromosomes , lagging chromosomes , disrupted chromosome segregation  and star cluster  chromosomes . with variable percentage of each type. This reflects the dangerous of these chemical compounds on mitotic cell division .
Keywords
Allium cepa; L . chromosomal aberrations; cyclophosphamide; cytogenetic effects; Ethyle methane sulphonate; Mitotic index
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