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Kassem, M., Hauka, F., Afify, A., El-Sawah, A. (2013). PRODUCTION AND PROPERTIES OF INULINASE FROM Arthrobacter sp.. Journal of Agricultural Chemistry and Biotechnology, 4(6), 239-249. doi: 10.21608/jacb.2013.53093
M. M. Kassem; F. I. A. Hauka; Aida H. Afify; A. M. El-Sawah. "PRODUCTION AND PROPERTIES OF INULINASE FROM Arthrobacter sp.". Journal of Agricultural Chemistry and Biotechnology, 4, 6, 2013, 239-249. doi: 10.21608/jacb.2013.53093
Kassem, M., Hauka, F., Afify, A., El-Sawah, A. (2013). 'PRODUCTION AND PROPERTIES OF INULINASE FROM Arthrobacter sp.', Journal of Agricultural Chemistry and Biotechnology, 4(6), pp. 239-249. doi: 10.21608/jacb.2013.53093
Kassem, M., Hauka, F., Afify, A., El-Sawah, A. PRODUCTION AND PROPERTIES OF INULINASE FROM Arthrobacter sp.. Journal of Agricultural Chemistry and Biotechnology, 2013; 4(6): 239-249. doi: 10.21608/jacb.2013.53093

PRODUCTION AND PROPERTIES OF INULINASE FROM Arthrobacter sp.

Article 2, Volume 4, Issue 6, June 2013, Page 239-249  XML PDF (412.06 K)
Document Type: Original Article
DOI: 10.21608/jacb.2013.53093
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Authors
M. M. Kassem; F. I. A. Hauka; Aida H. Afify; A. M. El-Sawah
Microbiol. Dept., Fac. Agric., Mansoura Univ., Egypt.
Abstract
Microbial inulinase has been proposed as the most promising approach to obtain fructose syrup from inulin which is used now, instead of sucrose, in many foods, pharmaceuticals, and beverages. Production and properties of inulinase from Arthrobacter sp. had been studied and the results revealed that, maximal yield of enzyme production is attained within 48 hours after inoculation, high inulinase production was observed when sucrose was used as additional carbon source, ammonium sulphate induced inulinase production by Arthrobacter sp., which was found as the best nitrogen source. Maximum inulinase production was obtained by using inoculum volume 5% (v/v), pH 7.0, at 30 0C. The properties of crude inulinase from Arthrobacter sp. was also tested. The enzyme showed maximum activity at pH 7.5 and 50 0C. The enzyme was stable more than 80% of its maximal activity at 30-50 0C after 30 min heat treatment.
Keywords
Arthrobacter sp; inulinase; inulin; hydrolysis; high fructose syrup
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