USING SOME AGRORESIDUES FOR AMYLASE PRODUCTION BY SOME LOCAL Bacillus ISOLATES IN SOLID STATE FERMENTATION

Selim, A. E. I.; Hauka, F. I. A.; Amer, Aida H. A.; Elattapy, A. M. M.

doi:10.21608/jacb.2011.57281

USING SOME AGRORESIDUES FOR AMYLASE PRODUCTION BY SOME LOCAL Bacillus ISOLATES IN SOLID STATE FERMENTATION

Document Type : Original Article

Authors

Microbiology dep., Fac. Agric., Mansoura Univ.

10.21608/jacb.2011.57281

Abstract

The production of extracellular amylases by some local Bacillus isolates was studied in solid state fermentation (SSF) using wheat bran (WB), rice bran (RB), corn bran (CB), potato waste (PW) individually as well as in combinations (ratio of 1:1 w/w). The appropriate incubation period was examined. Among the four substrates tested highest enzyme production was observed with WB by all Bacillus isolates. Maximum enzyme production (425.26 U/g) was observed with WB after 72 h by B. licheniformis and decreased with further incubation. Maximum enzyme production (384.27 U/g) was observed with RB after 96 h incubation period by B. licheniformis, while maximum enzyme production (396.53 U/g) was observed with CB by B. subtilis 10 at 72 h and maximum enzyme production (389.35U/g) was observed with PW by B. brevis 11 after 72 h. All combination treatments of substrates (ratio 1:1 w/w) gave significant decreases in the enzyme production compared to individual substrates. Although, RB, CB and PW were proved promising substitutes for wheat bran in amylase production.

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