Antagonistic Effect of Rhizobium, Bacillus, Pseudomonas, Trichoderma on Fusarium and Rhizocotonia Compared with Moncut In Vitro.

Document Type : Original Article

Authors

1 جامعة الأزهر- کلية الزراعة بالقاهرة- قسم النبات الزراعى فرع الميکروبيولوجى

2 وزارة الزراعة- مرکز البحوث الزراعية- معهد بحوث أمراض النبات

Abstract

This investigation was carried out in 2018 to study activity Rhizobium leguminosarum biovarphaseoli, Bacillus subtilis, Pseudomonas fluorescens,Trichoderma album and T. hamatum against of some soil pathogenic plant fungi; Fusarium  oxysporum and Rhizoctonia solani  causative agents  root rot disease of common bean was evaluated on culture medium. The bio-agent Rhizobium was checked as culture filtrate, different concentrations; 25, 50,100,150,200 and 250%, also by their cells as opposite to test fungi to observe effect on radial growth and inhibition zone percent by dual method compared with control. In addition bio agent effect was compared with effect of Moncut fungicide; different concentrations; 1, 10, 25, 50 and 100 ppm by agar well diffusion method.The obtained results revealed that bio - agents gave significance effect and maximum inhibition growth of Fusarium  oxysporium and Rhizoctonia solani  compared with control as well as Moncut fungicide. Overall rhizobia nitrogen fixing, growth regulators production, also gave inhibition growth of test fungi reached 89.50 , 90.29%  and in cultural filtrate conc.100% manner , but cells treatment were,59.96% and 38.86% in the previous order. 

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