Al-Ashkar, B., Al-Mariri, A., Hamad, I. (2010). DETECTION OF Brucella melitensis FROM FILTERED BOVINE PERIPHERAL-BLOOD BY POLYMERASE CHAIN REACTION TEST. Journal of Agricultural Chemistry and Biotechnology, 1(4), 229-234. doi: 10.21608/jacb.2010.89424
Buthaina Al-Ashkar; A. Al-Mariri; Ibtissam Hamad. "DETECTION OF Brucella melitensis FROM FILTERED BOVINE PERIPHERAL-BLOOD BY POLYMERASE CHAIN REACTION TEST". Journal of Agricultural Chemistry and Biotechnology, 1, 4, 2010, 229-234. doi: 10.21608/jacb.2010.89424
Al-Ashkar, B., Al-Mariri, A., Hamad, I. (2010). 'DETECTION OF Brucella melitensis FROM FILTERED BOVINE PERIPHERAL-BLOOD BY POLYMERASE CHAIN REACTION TEST', Journal of Agricultural Chemistry and Biotechnology, 1(4), pp. 229-234. doi: 10.21608/jacb.2010.89424
Al-Ashkar, B., Al-Mariri, A., Hamad, I. DETECTION OF Brucella melitensis FROM FILTERED BOVINE PERIPHERAL-BLOOD BY POLYMERASE CHAIN REACTION TEST. Journal of Agricultural Chemistry and Biotechnology, 2010; 1(4): 229-234. doi: 10.21608/jacb.2010.89424
DETECTION OF Brucella melitensis FROM FILTERED BOVINE PERIPHERAL-BLOOD BY POLYMERASE CHAIN REACTION TEST
1Dept. of Plant Biology, Faculty of Science, University of Damascus
2Dept. of Molecular Biology and Biotechnology, Atomic Energy Commission, Damascus
3Dept. of Environment, Faculty of Science, University of Damascus
Abstract
Brucella melitensis contaminants of bovine peripheral-blood can be concentrated and isolated by filtration; and their DNA can be released for subsequent PCR detection by heating the isolated colony at 95°C for 10 min. Detecting rate of brucellosis in the infected sample was less than 20 CFU/ml of blood, after leukocytes filtration and culturing, as well as on isolated colonies. This PCR method also enables us to make the diagnosing of brucellosis for the animals with a negative complement fixation test.
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