DETECTION OF Brucella melitensis FROM FILTERED BOVINE PERIPHERAL-BLOOD BY POLYMERASE CHAIN REACTION TEST

Al-Ashkar, Buthaina; Al-Mariri, A.; Hamad, Ibtissam

doi:10.21608/jacb.2010.89424

DETECTION OF Brucella melitensis FROM FILTERED BOVINE PERIPHERAL-BLOOD BY POLYMERASE CHAIN REACTION TEST

Document Type : Original Article

Authors

¹ Dept. of Plant Biology, Faculty of Science, University of Damascus

² Dept. of Molecular Biology and Biotechnology, Atomic Energy Commission, Damascus

³ Dept. of Environment, Faculty of Science, University of Damascus

10.21608/jacb.2010.89424

Abstract

Brucella melitensis contaminants of bovine peripheral-blood can be concentrated and isolated by filtration; and their DNA can be released for subsequent PCR detection by heating the isolated colony at 95^°C for 10 min. Detecting rate of brucellosis in the infected sample was less than 20 CFU/ml of blood, after leukocytes filtration and culturing, as well as on isolated colonies. This PCR method also enables us to make the diagnosing of brucellosis for the animals with a negative complement fixation test.

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