Extraction, Purification and Spectroscopic Characterization of Phycobiliproteins Extracted from some Nostoc Spp.

Hussein, Mervat; El-Naggar, Noura; El-Sawah, Asmaa

doi:10.21608/jacb.2017.38902

Extraction, Purification and Spectroscopic Characterization of Phycobiliproteins Extracted from some Nostoc Spp.

Document Type : Original Article

Authors

¹ Botany Dept., Fac. of Sci., Mansoura Univ., Mansoura, Egypt

² Dept. of Bioprocess Development, Genetic Engin. and Biotechnol. Res. Inst.,, City of Scientific Res. and Technol. Appl., Alexandria, Egypt

10.21608/jacb.2017.38902

Abstract

Phycocyanin and phycoerythrin are considered the major phycobiliprotein in many cyanobacteria as well as being a secondary phycobiliprotein in some red algae. In this article, the biomass of Nostoc linckia was harvested at the 16^th day, which approximately equalled 361 mg/L dry biomass for attaining the maximum content of phycocyanin, and the biomass of Nostoc carneum was collected at the 17^thday that approximately equalled 326 mg/L dry biomass for attaining the maximum content of phycoerythrin. Phycocyanin and phycoerythrin were extracted from both Nostoc linckia and Nostoc carneum respectively. They were extracted by successive cycles of freezing and thawing using 50 mM phosphate buffer solution (pH 7) then purified by single step of precipitation in 65 % of ammonium sulphate. Phycocyanin and phycoerythrin achieved 2.29 and 3.02 of purity ratio; respectively. The purified phycocyanin and phycoerythrin exhibited maximum absorbance at 614 and 560 nm respectively.

Keywords