El Morsi, A., El-Bendary, M., Neamatallah, M., Elsayed, H. (2018). Phylogenetic Analysis and Genotyping Distribution of Hepatitis C Virus Isolates Based on the 5`Untranslated Region among some Egyptian Populations. Journal of Agricultural Chemistry and Biotechnology, 9(1), 21-26. doi: 10.21608/jacb.2018.35151
adel El Morsi; mahmoud El-Bendary; mostafa Neamatallah; Heba Elsayed. "Phylogenetic Analysis and Genotyping Distribution of Hepatitis C Virus Isolates Based on the 5`Untranslated Region among some Egyptian Populations". Journal of Agricultural Chemistry and Biotechnology, 9, 1, 2018, 21-26. doi: 10.21608/jacb.2018.35151
El Morsi, A., El-Bendary, M., Neamatallah, M., Elsayed, H. (2018). 'Phylogenetic Analysis and Genotyping Distribution of Hepatitis C Virus Isolates Based on the 5`Untranslated Region among some Egyptian Populations', Journal of Agricultural Chemistry and Biotechnology, 9(1), pp. 21-26. doi: 10.21608/jacb.2018.35151
El Morsi, A., El-Bendary, M., Neamatallah, M., Elsayed, H. Phylogenetic Analysis and Genotyping Distribution of Hepatitis C Virus Isolates Based on the 5`Untranslated Region among some Egyptian Populations. Journal of Agricultural Chemistry and Biotechnology, 2018; 9(1): 21-26. doi: 10.21608/jacb.2018.35151
Phylogenetic Analysis and Genotyping Distribution of Hepatitis C Virus Isolates Based on the 5`Untranslated Region among some Egyptian Populations
1Botany Department, Faculty of Science, Mansoura University
2Tropical Medicine Department, Faculty of Medicine, Mansoura University
3Biochemistry Department, Faculty of Medicine, Mansoura University
Abstract
Hepatitis C virus (HCV) is a global problem and the genetic diversity in non-Western countries particularly in Egypt, where the highest prevalence of HCV is very important because of showing distribution of different HCV genotypes . This study aimed to report phylogenetic analysis of hepatitis C virus isolates based on the 5` untranslated region (5`UTR) in Egyptian populations using 51 samples with high and moderate viral load , selected from different regions in Egypt. Samples were identified based on RT-PCR amplification of the 5` UTR. The data were confirmed by sequencing and phylogenetic analysis using Multiple Sequence Alignment to describe the similarities of different isolates of HCV.The results appeared a high level of genetic diversity of genotype 4 with 90.2% that was commonly found with its subtypes ranged 4a (74.5%) ,4l (5.9%) ,4v (5.9%) and 4n (3.9%) while genotype 1 recorded 9.8% with its subtypes ranged 1a (3.9%) ,1b (2.0%) and 1g (3.9%). Using Multiple Sequence Alignment of HCV isolates indicated high percent of similarities between different genotypes of isolates. The results obtained in this study provide a strong evidence for high percent of similarities between different genotypes of isolates obtained from different Egyptian regions.
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