Hussein, E., Aly, A., El-Awamri, A., Habeb, M. (2018). Use of Serological Interactions to Differentiate between Trichoderma harzianum and Trichoderma longbrachiatum Isolated from Cotton Roots. Journal of Agricultural Chemistry and Biotechnology, 9(2), 67-75. doi: 10.21608/jacb.2018.35162
ezat Hussein; ali Aly; ahmed El-Awamri; Marian Habeb. "Use of Serological Interactions to Differentiate between Trichoderma harzianum and Trichoderma longbrachiatum Isolated from Cotton Roots". Journal of Agricultural Chemistry and Biotechnology, 9, 2, 2018, 67-75. doi: 10.21608/jacb.2018.35162
Hussein, E., Aly, A., El-Awamri, A., Habeb, M. (2018). 'Use of Serological Interactions to Differentiate between Trichoderma harzianum and Trichoderma longbrachiatum Isolated from Cotton Roots', Journal of Agricultural Chemistry and Biotechnology, 9(2), pp. 67-75. doi: 10.21608/jacb.2018.35162
Hussein, E., Aly, A., El-Awamri, A., Habeb, M. Use of Serological Interactions to Differentiate between Trichoderma harzianum and Trichoderma longbrachiatum Isolated from Cotton Roots. Journal of Agricultural Chemistry and Biotechnology, 2018; 9(2): 67-75. doi: 10.21608/jacb.2018.35162
Use of Serological Interactions to Differentiate between Trichoderma harzianum and Trichoderma longbrachiatum Isolated from Cotton Roots
1Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt
2Botany Department, Faculty of Science, Ain Shams University, Cairo, Egypt
Abstract
Double diffusion (DD) and immunoelectrophoresis (IE) techniques were used to differentiate among Trichoderma isolates. Each of T. longbrachiatum and T. harzianum isolates tended to group together based on their antigenic composition, which indicated a remarkable overall serological similarity among isolates of each species. However, the serological differences among the isolates did not always reflect the taxonomic differences because some isolates of T. longbrachiatum and T. harzianum showed considerable serological similarity. Thus, DD and IE were not useful techniques in identification of Trichoderma isolates.
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