Antioxidant and Antimicrobial Activities of Echinacea purpurea L. Extracts. الانشطة المضادة للأکسدة والمیکروبات فی مستخلصات نبات القنفدیة الارجوانیة

Hassan, R. A.; Ali, Safaa M.; Mohamed, Azza H.; Hamed, Sahar E.

doi:10.21608/jacb.2020.123553

Antioxidant and Antimicrobial Activities of Echinacea purpurea L. Extracts. الانشطة المضادة للأکسدة والمیکروبات فی مستخلصات نبات القنفدیة الارجوانیة

Document Type : Original Article

Authors

¹ Fac. of Agric.,Mansoura University

² Fac. of Agric., Damietta University

10.21608/jacb.2020.123553

Abstract

Echinacea purpurea L. (E. purpurea) is one of the most important medical herbs belongs to Asteraceae family. This plant contains many chemical compounds. It has also demonstrated numerous biological activities as both antioxidant and antibacterial. Methanol was used to extract green plant sample and consider as crud extract. This last one was partitioned into four solvents i.e. petrolium ether, CH₂Cl₂, C₂H₅COOCH₃ and C₄H₉OH . The obtained fractions extracts were tested for their total phenolic content (TPC), total flavonoid content (TFC). Antioxidant activities were carried out using three methods I.e phosphomolybdenum, reducing power and radical assay DPPH. Crude methanolic extract was subjected to six microbial strains included fungi, yeast and bacteria i.e: Aspergillus oryzae , Candida albicans, Saccharomyces cerevisiae, Alternaia solani and Escherichia coli (G^-), Streptococcus faecalis (G⁺). E. purpurea extracts had the highest effect of on yeast (Saccharomyces cerevisiae) with average value of 15.0 mm 100 ppm extract. Results showed that the highest concentration of TPC for ethyl acetate extract was 90.6 mg GAE/g dw wheras pet. ether gave the lowest value of 33.83 mg GAE/g d.w. TFC was ranged from 146.53 to 322.23mg QE/g dw for ethyl acetate and methanol extracts, respectively. Butanol extract showed the highest antioxidant activity with average value of 70.22 μg/ml.

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